π–π stacked DNA G-wire nanostructures formed by a short G-rich oligonucleotide containing a 3′–3′ inversion of polarity site,Organic Chemistry Frontiers

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π–π stacked DNA G-wire nanostructures formed by a short G-rich oligonucleotide containing a 3′–3′ inversion of polarity site
Organic Chemistry Frontiers ( IF 4.6 ) Pub Date : 2020-07-06 , DOI: 10.1039/d0qo00561d
Maria Marzano _{1,

2,

3,

4} , Andrea P. Falanga _{1,

2,

3,

4} , Principia Dardano _{3,

4,

5,

6} , Stefano D'Errico _{2,

4,

7,

8} , Ilaria Rea _{3,

4,

5,

6} , Monica Terracciano _{1,

2,

3,

4} , Luca De Stefano _{3,

4,

5,

6} , Gennaro Piccialli _{1,

2,

3,

4} , Nicola Borbone _{1,

2,

3,

4} , Giorgia Oliviero _{2,

3,

4,

9}

Affiliation

The interest in DNA based nanostructures arises from their potential applications in diagnostics and drug delivery and in the development of new hybrid and conducting materials. Guanine-rich oligonucleotides can multimerize forming long and stable supramolecular structures, known as G-wires, based on the G-quadruplex (G4) motif. Herein, we report a method to easily obtain long DNA G-wires based on a new tetramolecular G4 subunit formed by the d(5′-CGG-3′-3′-GGC-5′) sequence containing a 3′–3′ inversion of polarity site. The formation of the G-wire assembly exploits the multimerization ability of G4s presenting the CGG motif at their 5′ end via π–π stacking interactions between flanking G4 subunits. The structures and the stability of the resulting G-wires were investigated by HPLC, size exclusion chromatography, polyacrylamide gel electrophoresis, circular dichroism, ¹H NMR and atomic force microscopy studies.

中文翻译：

由短的富含G的寡核苷酸形成的π–π堆叠的DNA G线纳米结构，其中包含极性位点的3'–3'倒置

对基于DNA的纳米结构的兴趣来自它们在诊断和药物输送以及新的杂化和导电材料开发中的潜在应用。富含鸟嘌呤的寡核苷酸可以多聚形成基于G四链体（G4）的长而稳定的超分子结构，称为G线。本文中，我们报道了一种基于由包含3'–3'的d（5'-CGG-3'-3'-GGC-5'）序列形成的新的四分子G4亚基，轻松获得长DNA G线的方法极性位点反转。G线组件的形成利用了G4的5'端通过G4亚基之间的π–π堆积相互作用。通过HPLC，尺寸排阻色谱，聚丙烯酰胺凝胶电泳，圆二色性，¹ H NMR和原子力显微镜研究，研究了所得G线的结构和稳定性。

更新日期：2020-08-14

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