The point of the present investigation was to blend effective chitosan nanoparticles (CNPs) loaded with Pterocarpus marsupium (PM) heartwood extract and evaluate its biomedical applications. Various plant extract concentrations (PM-CNPs-1, PM-CNPs-2, PM-CNPs-3) are used to synthesize chitosan nanoparticles and optimized to acquire a stable nanoparticle formulation. The entrapment efficiency and in vitro release studies of the plant extract encapsulated in CNPs are estimated. The PM-loaded CNPs were characterized by X-ray diffraction, dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The synthesized chitosan nanoparticles were evaluated for their alpha-amylase inhibitory activity and inhibition of albumin denaturation activity. The XRD pattern of PM-CNPs shows less number of peaks at low intensity due to the interaction of chitosan with sodium tripolyphosphate. The FT-IR spectrum with peaks at 1639.55 and 1149.02 cm−1 confirms the formation of chitosan nanoparticles. The size of the nanoparticles ranges between 100 and 110 nm with spherical shape illustrated by SEM and TEM analysis. The nanoparticle formulation with 10% plant extract concentration (PM-CNPs-2) showed optimum particle size, higher stability, enhanced entrapment efficiency, and sustained drug release characteristics. Synthesized chitosan nanoparticles have shown a significant increase in alpha-amylase inhibition and appreciable anti-inflammatory activity as measured by inhibition of protein denaturation. The investigation reports the eco-friendly, cost-effective method for synthesizing chitosan nanoparticles loaded with Pterocarpus marsupium Rox.b heartwood extract.

中文翻译：

---

紫檀丸Roxb。心材提取物合成壳聚糖纳米粒子及其生物医学应用。

本研究的重点是掺入有效负载壳聚糖纳米粒（CNP）的紫檀（PM）心材提取物，并评估其生物医学应用。各种植物提取物浓度（PM-CNPs-1，PM-CNPs-2，PM-CNPs-3）用于合成壳聚糖纳米粒子，并进行了优化以获得稳定的纳米粒子配方。估计了封装在CNP中的植物提取物的包封效率和体外释放研究。通过X射线衍射，动态光散射（DLS），傅立叶变换红外光谱（FT-IR），扫描电子显微镜（SEM）和透射电子显微镜（TEM）对PM负载的CNP进行了表征。评价了合成的壳聚糖纳米颗粒的α-淀粉酶抑制活性和白蛋白变性活性的抑制。由于壳聚糖与三聚磷酸钠的相互作用，PM-CNP的XRD图谱在低强度下显示较少的峰数。FT-IR光谱的峰在1639.55和1149.02 cm-1处确认了壳聚糖纳米颗粒的形成。纳米颗粒的尺寸在100至110nm之间，具有通过SEM和TEM分析示出的球形。具有10％植物提取物浓度（PM-CNPs-2）的纳米颗粒制剂显示最佳粒径，更高的稳定性，增强的包封效率和持续的药物释放特性。合成的壳聚糖纳米颗粒显示出对α-淀粉酶的抑制作用显着增加，并且通过抑制蛋白质变性来测量其具有明显的抗炎活性。调查报告表明，