Antimicrobial peptides play important roles in both plant and animal defense systems. Moreover, over-expression of CaAMP1 (Capsicum annuum antimicrobial protein 1), an antimicrobial protein gene isolated from C. annuum leaves infected with Xanthomonas campestris pv. vesicatoria, confers broad-spectrum resistance to hemibiotrophic bacterial and necrotrophic fungal pathogens in Arabidopsis. Phytophthora root and stem rot (PRR), caused by the fungus Phytophthora sojae, is one of the most devastating diseases affecting soybean (Glycine max) production worldwide. In this study, CaAMP1 was transformed into soybean by Agrobacterium-mediated genetic transformation. Integration of the foreign gene in the genome of transgenic soybean plants and its expression at the translation level were verified by Southern and western blot analyses, respectively. CaAMP1 over-expression (CaAMP1-OX) lines inoculated with P. sojae race 1 exhibited enhanced and stable PRR tolerance through T2–T4 generations compared with the wild-type Williams 82 plants. Gene expression analyses in the transgenic plants revealed that the expression of salicylic acid-dependent, jasmonic acid-dependent, and plant disease resistance genes (R-genes) were significantly up-regulated after P. sojae inoculation. These results indicate that CaAMP1 over-expression can significantly enhance PRR tolerance in soybean by eliciting resistance responses mediated by multiple defense signaling pathways. This provides an alternative approach for developing soybean varieties with improved tolerance against soil-borne pathogenic PRR.

中文翻译：

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通过在大豆中过度表达植物抗菌肽 CaAMP1 基因，增强对疫霉根腐病和茎腐病的耐受性。


抗菌肽在植物和动物防御系统中发挥着重要作用。此外，CaAMP1（辣椒抗菌蛋白 1）的过度表达，这是一种从感染黄单胞菌 pv. 的辣椒叶中分离出来的抗菌蛋白基因。 vesicatoria，赋予拟南芥中半生物营养细菌和坏死营养真菌病原体的广谱抗性。疫霉根腐病 (PRR) 由真菌大豆疫霉 (Phytophthora sojae) 引起，是影响全球大豆 (Glycine max) 生产的最具破坏性的病害之一。在这项研究中，CaAMP1 通过农杆菌介导的遗传转化转化到大豆中。外源基因在转基因大豆植物基因组中的整合及其在翻译水平的表达分别通过Southern印迹和western印迹分析得到验证。与野生型 Williams 82 植物相比，接种酱油小种 1 的 CaAMP1 过表达 (CaAMP1-OX) 品系在 T2-T4 代中表现出增强且稳定的 PRR 耐受性。转基因植物中的基因表达分析表明，接种酱油后，水杨酸依赖性、茉莉酸依赖性和植物抗病基因（R基因）的表达显着上调。这些结果表明，CaAMP1 过表达可以通过引发多种防御信号通路介导的抗性反应，显着增强大豆的 PRR 耐受性。这为开发对土传致病性PRR具有更高耐受性的大豆品种提供了一种替代方法。