当前位置：
X-MOL 学术
›
Anal. Methods
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
A spot test for total esters determination in sugarcane spirits exploiting smartphone-based digital images.
Analytical Methods ( IF 2.7 ) Pub Date : 2020-07-06 , DOI: 10.1039/d0ay01013h Samara Soares 1 , Karoline R R Campos , Wanessa R Melchert , Fábio R P Rocha
Analytical Methods ( IF 2.7 ) Pub Date : 2020-07-06 , DOI: 10.1039/d0ay01013h Samara Soares 1 , Karoline R R Campos , Wanessa R Melchert , Fábio R P Rocha
Affiliation
|
Cachaça is an alcoholic beverage produced from sugarcane, whose flavor and taste are related to the esters content, usually expressed as equivalent to ethyl acetate. The official method for the determination of specific esters in cachaça is based on gas chromatography with a flame ionization detector, whereas a volumetric procedure is recommended for determining the total content. Because of the high analytical demand, faster and more practical analytical procedures are required for quality control of the product. The aim of this work was to develop a spot test exploiting smartphone-based digital images for in situ determination of total esters in cachaça. The procedure was based on the reaction of the analytes with hydroxylamine, generating the corresponding hydroxamate ions, which form violet complexes with Fe(III) in an acidic medium. Digital images were acquired under controlled illumination and converted to RGB values using the PhotoMetrix® 1.8 application. The values of channel B were taken as the analytical response because of the complementarity with the color of the reaction product. A linear response was obtained within 100–500 mg L−1 ethyl acetate, with the coefficient of variation (n = 10) and limit of detection (99.7% confidence level) estimated at 1.1% and 30 mg L−1 ethyl acetate, respectively. The procedure consumes only 1.4 mg NH2OH·HCl and 115 μg Fe(III) and generates only 900 μL of waste per determination. The results of the proposed procedure agreed with those obtained by the reference volumetric method at the 95% confidence level.
中文翻译：
利用基于智能手机的数字图像对甘蔗酒中总酯含量进行的现场测试。
Cachaça是一种由甘蔗生产的酒精饮料，其风味和味道与酯含量有关，通常表示为乙酸乙酯。测定酒渣中特定酯类的官方方法是基于带有火焰离子化检测器的气相色谱法，但是建议采用体积法测定总含量。由于对分析的需求很高，因此需要更快，更实用的分析程序来控制产品的质量。这项工作的目的是开发一个现场测试开发基于智能手机的数字图像进行现场茶中总酯的测定。该程序基于分析物与羟胺的反应，生成相应的异羟肟酸根离子，这些离子在酸性介质中与Fe（III）形成紫色络合物。在受控照明下获取数字图像，并使用PhotoMetrix®1.8应用程序将其转换为RGB值。由于与反应产物的颜色互补，因此将通道B的值作为分析响应。在100–500 mg L -1乙酸乙酯内获得线性响应，变异系数（n = 10）和检出限（99.7％置信水平）估计为1.1％和30 mg L -1分别为乙酸乙酯。该程序仅消耗1.4 mg NH 2 OH·HCl和115μgFe（III），每次测定仅产生900μL废物。拟议程序的结果与参考体积法在95％置信水平下获得的结果相符。
更新日期：2020-08-14
中文翻译：
利用基于智能手机的数字图像对甘蔗酒中总酯含量进行的现场测试。
Cachaça是一种由甘蔗生产的酒精饮料，其风味和味道与酯含量有关，通常表示为乙酸乙酯。测定酒渣中特定酯类的官方方法是基于带有火焰离子化检测器的气相色谱法，但是建议采用体积法测定总含量。由于对分析的需求很高，因此需要更快，更实用的分析程序来控制产品的质量。这项工作的目的是开发一个现场测试开发基于智能手机的数字图像进行现场茶中总酯的测定。该程序基于分析物与羟胺的反应，生成相应的异羟肟酸根离子，这些离子在酸性介质中与Fe（III）形成紫色络合物。在受控照明下获取数字图像，并使用PhotoMetrix®1.8应用程序将其转换为RGB值。由于与反应产物的颜色互补，因此将通道B的值作为分析响应。在100–500 mg L -1乙酸乙酯内获得线性响应，变异系数（n = 10）和检出限（99.7％置信水平）估计为1.1％和30 mg L -1分别为乙酸乙酯。该程序仅消耗1.4 mg NH 2 OH·HCl和115μgFe（III），每次测定仅产生900μL废物。拟议程序的结果与参考体积法在95％置信水平下获得的结果相符。
京公网安备 11010802027423号