Herbal tea with antioxidant ingredients has gained increasing attention in the field of functional foods due to their amelioration potential in aging-related diseases. Wanglaoji herbal tea (WHT) is a kind of traditional beverage made from herbal materials. This study was performed to investigate its antioxidant activity and identify its protective effect on a H₂O₂-induced cell damage model. In this study, we identified six kinds of phenolic acids with antioxidant activity in WHT, among which rosmarinic acid had the highest content and the highest contribution ratio to the antioxidant activity of WHT. Moreover, compared with the H₂O₂-induced damage group, the WHT treatment group can significantly increase the viability of cells and decrease the ratio of senescence-associated β-galactosidase-positive cells, intracellular malondialdehyde levels, and the percentage of G1 phase. Furthermore, enrichment analysis of differentially expressed genes revealed that heme oxygenase1 (HMOX1) was a key gene for protective effect of WHT on oxidative stress-induced cell damage. Thus, WHT exerted protective effects not only by scavenging reactive oxygen species but also by inducing the expression of cytoprotective genes by activating the HMOX1 pathway, which showed that WHT had a potential of promoting health by reducing oxidative stress-induced cell damage.

中文翻译：

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激活血红素加氧酶1途径的凉茶对H2O2诱导的氧化损伤的抗氧化作用和细胞保护潜力。

具有抗氧化剂成分的凉茶由于其改善衰老相关疾病的潜力而在功能性食品领域引起了越来越多的关注。王老吉凉茶（WHT）是一种由草药制成的传统饮料。进行该研究以研究其抗氧化活性并鉴定其对H ₂ O ₂诱导的细胞损伤模型的保护作用。本研究确定了六种在WHT中具有抗氧化活性的酚酸，其中迷迭香酸含量最高，对WHT的抗氧化活性的贡献率最高。而且，与H ₂ O _2相比诱导损伤组，WHT治疗组可显着提高细胞活力，降低衰老相关β-半乳糖苷酶阳性细胞比例，细胞内丙二醛水平和G1期百分比。此外，差异表达基因的富集分析表明，血红素加氧酶1（HMOX1）是WHT对氧化应激诱导的细胞损伤的保护作用的关键基因。因此，WHT不仅可以通过清除活性氧来发挥保护作用，还可以通过激活HMOX1途径诱导细胞保护基因的表达来发挥保护作用，这表明WHT可以通过减少氧化应激诱导的细胞损伤来促进健康。