Purpose. The effect of preoperative anesthesia on coronary artery bypass grafting without extracorporeal circulation is not apparent. We want to investigate the effects and molecular mechanisms of two anesthesia methods on the treatment of coronary artery bypass grafting (OPCABG) under extracorporeal circulation. Patients and Methods. The data of inhaled anesthesia and intravenous anesthesia before coronary artery bypass grafting were downloaded from the GEO database, and the differences were analyzed with the control group. The combination of multiple analytical methods can decipher the mechanism of anesthesia on surgery, including protein interaction network analysis, enrichment analysis, and regulatory subprediction. Results. This study obtained 6699 differential genes under two kinds of anesthesia before OPCABG. By constructing a protein interaction network of differentially expressed genes, we obtained 14 functional module networks. By predicting regulators of functional module genes, we revealed a series of ncRNAs (miR-129-5p, miR-340-5p, and miR-410-3p) and transcription factors (VHL and YBX1). Conclusion. Based on functional module network analysis, we identified the effects of preoperative inhalation anesthesia and intravenous anesthesia on OPCABG, which provides a valuable theoretical reference for subsequent clinical studies.

中文翻译：

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非体外循环冠状动脉旁路移植术中不同表达基因及其功能模块的综合研究。

目的。术前麻醉对无体外循环冠状动脉旁路移植术的影响不明显。我们想研究两种麻醉方法对体外循环下冠状动脉旁路移植术（OPCABG）治疗的影响和分子机制。患者和方法。从GEO数据库下载冠状动脉搭桥术前吸入麻醉和静脉麻醉数据，并与对照组进行差异分析。多种分析方法的结合可以破译麻醉对手术的作用机制，包括蛋白质相互作用网络分析、富集分析和调节亚预测。结果. 本研究在OPCABG前两种麻醉下获得了6699个差异基因。通过构建差异表达基因的蛋白质相互作用网络，我们获得了14个功能模块网络。通过预测功能模块基因的调节因子，我们揭示了一系列 ncRNA（miR-129-5p、miR-340-5p 和 miR-410-3p）和转录因子（VHL 和 YBX1）。结论。基于功能模块网络分析，确定术前吸入麻醉和静脉麻醉对OPCABG的影响，为后续临床研究提供有价值的理论参考。