Abstract

In recent years, a great deal of attention has been paid to the combined use of multiple antitumor drugs for better cancer treatment. The aims of the study are to construct a nanoparticle drug delivery system for the co-delivery of irinotecan hydrochloride and curcumin and to develop an analytical method for simultaneously quantifying these molecules, which is essential for further studies of the co-delivered nano system. The irinotecan hydrochloride and curcumin co-delivered nanoparticle (ICN) were prepared by combinatorially entrapping them into polyethylene glycol–poly lactic acid-co-glycolic acid (PEG–PLGA) polymeric nanoparticles. A simple, sensitive and rapid high-performance liquid chromatography method was developed and validated to simultaneously quantify the compounds in the co-delivered nanoparticle system. Acetonitrile and ultrapure water containing sodium dodecyl sulfate (0.08 mol/L), disodium phosphate (Na₂HPO₄, 0.002 mol/L) and acetic acid (4%, v/v) were used as the mobile phase and their ratio was set at 50:50. The flow rate was set to 1.0 mL/min, and the temperature in the column oven was maintained at 40°C. The analysis was carried out at 256 and 424 nm to assess irinotecan hydrochloride and curcumin, respectively. Detectors with only one channel can also visualize both analytes in one chromatogram at 379 nm and still demonstrate acceptable sensitivity. The retention times for irinotecan hydrochloride and curium were 3.317 and 5.560 min, respectively. The method developed was confirmed to be sensitive, accurate (recovery, 100 ± 2%), precise (relative standard deviation, RSD ≤ 1%), robust and linear (R² ≥ 0.9996) in the range from 2.05 to 1050 μg/mL. The presented method has been used to quantify irinotecan hydrochloride and curcumin in the co-delivered ICN nano system to assess the drug delivery quality of the nanoparticles and can also be used for routine analysis because of its simplicity and accuracy.

中文翻译：

---

同时测定共同递送的聚合物纳米颗粒中盐酸伊立替康和姜黄素的快速，高效液相色谱方法。

摘要

近年来，将多种抗肿瘤药物联合用于更好的癌症治疗已引起了极大的关注。该研究的目的是构建用于共同递送伊立替康盐酸盐和姜黄素的纳米颗粒药物递送系统，并开发一种用于同时定量这些分子的分析方法，这对于进一步研究共同递送的纳米系统至关重要。伊立替康盐酸盐和姜黄素共递送纳米颗粒（ICN）的制备方法是将它们组合包裹在聚乙二醇-聚乳酸-乙醇酸共聚物（PEG-PLGA）聚合物纳米颗粒中。开发并验证了一种简单，灵敏，快速的高效液相色谱方法，可同时定量测定共同提供的纳米颗粒系统中的化合物。_使用2 HPO ₄（0.002 mol / L）和乙酸（4％，v / v）作为流动相，其比例设定为50:50。流速设定为1.0mL / min，并且柱箱中的温度保持在40℃。在256和424 nm处进行了分析，分别评估了盐酸伊立替康和姜黄素。仅具有一个通道的检测器还可以在379 nm的一张色谱图中显示两种分析物，并且仍然显示出可接受的灵敏度。伊立替康盐酸盐和cur的保留时间分别为3.317和5.560 min。已证实开发的方法灵敏，准确（回收​​率100±2％），精确（相对标准偏差，RSD≤1％），稳健且线性（R ² ≥0.9996），范围为2.05至1050μg/ mL。所提出的方法已用于定量测定共递送的ICN纳米系统中的盐酸伊立替康和姜黄素，以评估纳米颗粒的药物递送质量，并且由于其简单性和准确性也可用于常规分析。