Abstract The vermicompost collected from dewatered domestic sludge as packing material in biofilter was investigated for hydrogen sulfide (H2S) removal. No nutrients or microbial inoculation was added throughout the experiment. The corresponding bacterial community characteristics in the vermicompost biofilter of different spatial levels were evaluated by Miseq high-throughput sequencing technique. The results showed that the vermicompost biofilter performed well during operation. The H2S removal efficiency reached nearly 100% under condition of the inlet concentration <350 mg m−3 and 0.25−0.35 m3 h−1 gas flow rate. The maximum elimination capacity of 20.2 g m−3 h−1 was observed at a flow rate of 0.35 m3 h−1. Furthermore, the amounts of biodegraded products and pH varied accordingly. In addition, the results from high-throughput sequencing revealed pronouncedly spatial variation of the vermicompost, and the Rhodanobacter, Halothiobacillus, Mizugakiibacter as well as Thiobacillus, which can play an important role in removing H2S, were predominant in the final vermicompost. These results imply that the vermicompost with diverse microbial communities has a good potential for eliminating H2S.

中文翻译：

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蚯蚓粪生物滤池去除H2S及细菌群落分析

摘要 研究了从脱水生活污泥中收集的蚯蚓堆作为生物过滤器的填料，以去除硫化氢（H2S）。在整个实验过程中没有添加任何营养物质或微生物接种。通过Miseq高通量测序技术评估了不同空间水平的蚯蚓粪生物滤池中相应的细菌群落特征。结果表明，蚯蚓粪生物滤池在运行过程中表现良好。在入口浓度<350 mg m-3 和0.25-0.35 m3 h-1 气体流量的条件下，H2S 去除效率接近100%。在 0.35 m3 h-1 的流速下观察到 20.2 gm-3 h-1 的最大消除能力。此外，生物降解产物的数量和 pH 值也相应变化。此外，高通量测序结果显示，蚯蚓粪空间变异明显，在最终的蚯蚓粪中，红丹杆菌、盐硫杆菌、水沟杆菌和硫杆菌在去除H2S方面起重要作用。这些结果意味着具有不同微生物群落的蚯蚓粪具有消除 H2S 的良好潜力。