The impacts of enzyme pre-treatments on anaerobic digestion of lignocellulosic biomass were explored by using corn straw as a substrate for enzyme pre-treatment and anaerobic digestion and by utilizing starch and microcrystalline cellulose as substrates for comparative analysis. The cellulase pre-treatment effectively improved the enzymatic hydrolysis of cellulose, decreased the crystallinity, and consequently showed 33.2% increase in methane yield. The methane yield of starch increased by 16.0% through amylase pre-treatment. However, when the substrate was corn straw, both the efficiencies of enzymes and methane production were markedly reduced by the lignocellulosic structure. The corn straw’s methane yields were 277.6 and 242.4 mL·CH 4 /g·VS with cellulase and amylase pre-treatment, respectively, which was 11.7% and 27.9% higher than that of the untreated corn straw. It may imply that the lignocellulose should be broken up firstly, enzyme pre-treatments could have great potentials when combined with other methods.

中文翻译：

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纤维素酶和淀粉酶对玉米秸秆厌氧消化酶水解和甲烷产生的影响

以玉米秸秆为底物进行酶预处理和厌氧消化，以淀粉和微晶纤维素为底物进行比较分析，探讨了酶预处理对木质纤维素生物质厌氧消化的影响。纤维素酶预处理有效地改善了纤维素的酶水解，降低了结晶度，从而使甲烷产率提高了33.2%。通过淀粉酶预处理，淀粉的甲烷产率提高了16.0%。然而，当基质为玉米秸秆时，木质纤维素结构会显着降低酶的效率和甲烷的产生。用纤维素酶和淀粉酶预处理玉米秸秆的甲烷产量分别为277.6和242.4 mL·CH 4 /g·VS，分别为11.7%和27。比未经处理的玉米秸秆高 9%。这可能意味着木质纤维素应该首先被分解，酶预处理与其他方法相结合可能具有很大的潜力。