Cannabis and its secondary metabolite content have recently seen a surge in research interest. Cannabis terpenes and terpenoids in particular are increasingly the focus of research efforts due to the possibility of their contribution to the overall therapeutic effect of medicinal cannabis. Current methodology to quantify terpenes in cannabis biomass mostly relies on large quantities of biomass, long extraction protocols, and long GC gradient times, often exceeding 60 min. They are therefore not easily applicable in the high-throughput environment of a cannabis breeding program. The method presented here, however, is based on a simple hexane extract from 40 mg of biomass, with 50 μg/mL dodecane as internal standard, and a gradient of less than 30 min. The method can detect 48 individual terpenes and terpenoids and was validated for selectivity, linearity, LOD/LOQ, precision, intermediate precision, and accuracy (recovery) for 22 terpenes and terpenoids. The validation parameters are comparable to previously published studies that employ significantly longer runtimes and/or more complex extraction protocols. It is currently being applied to medicinal cannabis precision breeding programs.

中文翻译：

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全面分析药用大麻生物量中萜类和萜类化合物的高通量方法。

大麻及其次级代谢产物的含量近来在研究兴趣方面激增。由于大麻萜类化合物和萜类化合物可能对药用大麻的整体治疗作用做出贡献，因此，大麻萜类化合物和萜类化合物尤其日益成为研究工作的重点。目前用于量化大麻生物质中萜烯含量的方法主要依赖于大量生物质，较长的提取流程和较长的GC梯度时间，通常超过60分钟。因此，它们不容易应用于大麻育种计划的高通量环境。但是，此处介绍的方法基于从40 mg生物质中以50μg/ mL十二烷为内标物的简单己烷提取物，且梯度小于30分钟。该方法可以检测48种单独的萜烯和萜类化合物，并经过了选择性验证，线性，LOD / LOQ，精度，中间精度和22个萜烯和萜类化合物的精度（回收率）。验证参数与以前发表的研究相当，后者采用更长的运行时间和/或更复杂的提取协议。目前正在将其应用于药用大麻精确育种计划。