Evaluation of the efficiency of using Salmonella Kentucky and Escherichia coli O119 bacteriophages in the treatment and prevention of salmonellosis and colibacillosis in broiler chickens,Letters in Applied Microbiology

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Evaluation of the efficiency of using Salmonella Kentucky and Escherichia coli O119 bacteriophages in the treatment and prevention of salmonellosis and colibacillosis in broiler chickens
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-07-20 , DOI: 10.1111/lam.13347
H K Sorour ₁ , A F Gaber ₁ , R A Hosny ₁

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Phage therapy is considered an alternative modality in the treatment of different bacterial diseases. However, their therapeutic and preventive roles against infections caused by Salmonella Kentucky and Escherichia coli O119 were of little attention. In this study, two phages were isolated, characterized and assessed for their potential therapeutic and preventive roles against S. Kentucky and E. coli O119 infections in broilers. Commercial 1‐day‐old arboacres broiler chicks were assigned to seven groups: Group Ӏ was as a negative control, groups (П and Ш) were assigned as positive controls by the challenge of S. Kentucky and E. coli O119, respectively. The remaining four groups (IV, V, VI and VII) were administrated with five repeated phage doses to determine the effect of multiple doses. Phages were administrated in groups (IV and VI) after challenging with S. Kentucky and E. coli O119, respectively to assess their therapeutic role; moreover, their preventive role was evaluated through administration in groups (V and VII) before challenging with S. Kentucky and E. coli O119, respectively. Sampling was done from different organs at three time points and revealed that phage‐treated groups had lower colony forming units of S. Kentucky and E. coli. Our results suggest that bacteriophages are efficient in the treatment and prevention of salmonellosis and colibacillosis in broiler farms.

中文翻译：

使用肯塔基沙门氏菌和大肠杆菌O119噬菌体治疗和预防肉鸡沙门氏菌病和大肠杆菌病的有效性评价

噬菌体疗法被认为是治疗不同细菌疾病的另一种方式。然而，它们对由肯塔基沙门氏菌和大肠杆菌O119 引起的感染的治疗和预防作用很少受到关注。在这项研究中，分离、表征和评估了两种噬菌体，它们对肉鸡中的 S. Kentucky 和 E. coli O119 感染具有潜在的治疗和预防作用。商业 1 日龄 arboacres 肉鸡被分配到 7 个组： Ӏ 组作为阴性对照，组（П 和 Ш）分别作为阳性对照，分别通过 S. Kentucky 和 E. coli O119 的挑战。其余四组 (IV、V、VI 和 VII) 以五次重复的噬菌体剂量给药，以确定多次剂量的效果。噬菌体在分别用 S. Kentucky 和 E.coli O119 攻击后按组（IV 和 VI）给药，以评估它们的治疗作用；此外，在分别用 S. Kentucky 和 E.coli O119 挑战之前，通过在组（V 和 VII）中给药来评估它们的预防作用。在三个时间点从不同器官进行采样，结果表明噬菌体处理组具有较低的 S. Kentucky 和大肠杆菌菌落形成单位。我们的结果表明，噬菌体可有效治疗和预防肉鸡养殖场的沙门氏菌病和大肠杆菌病。在三个时间点从不同器官进行采样，结果表明噬菌体处理组具有较低的 S. Kentucky 和大肠杆菌菌落形成单位。我们的结果表明，噬菌体可有效治疗和预防肉鸡养殖场的沙门氏菌病和大肠杆菌病。在三个时间点从不同器官进行采样，结果表明噬菌体处理组具有较低的 S. Kentucky 和大肠杆菌菌落形成单位。我们的结果表明，噬菌体可有效治疗和预防肉鸡养殖场的沙门氏菌病和大肠杆菌病。

更新日期：2020-07-20

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