Availability of Hydrogen Shapes the Microbial Abundance in Biofilm Anodes based on Geobacter Enrichment,ChemElectroChem

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Availability of Hydrogen Shapes the Microbial Abundance in Biofilm Anodes based on Geobacter Enrichment
ChemElectroChem ( IF 3.5 ) Pub Date : 2020-07-06 , DOI: 10.1002/celc.202000731
Benjamin Korth ₁ , Anne Kuchenbuch ₁ , Falk Harnisch ₁

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Biofilm anodes based on Geobacter enrichment in one‐chamber and two‐chamber reactors are qualitatively and quantitatively investigated. The methanogenic community of biofilms in both types of reactors consists exclusively of hydrogenotrophs, mainly the genus Methanobacterium. As qPCR demonstrates in one‐chamber reactors, the abundance of Geobacter and methanogens increases due to the presence of cathodically produced hydrogen. In two‐chamber reactors, the abundance of methanogens is decreased by 98 % compared to one‐chamber reactors. Adding hydrogen gas to the anodic compartment of two‐chamber reactors recovers the abundance of methanogens and Geobacter. Ratios of methanogens/bacteria are confirmed by using the cofactor F₄₂₀ autofluorescence of methanogens. The impact of the reactor setup for developing primary microbial electrochemical technologies is discussed, reasoning that two‐chamber reactors have to be generally favored. Yet, their use inheres a trade‐off when cultivating biofilm anodes, as the indicated syntrophic interactions between methanogens and Geobacter are reduced, but pH decrease might influence microbial processes.

中文翻译：

氢的可用性塑造了基于地细菌富集的生物膜阳极中微生物的丰度

对一室和两室反应器中基于地球细菌富集的生物膜阳极进行了定性和定量研究。在两种类型的反应器中，生物膜的产甲烷菌群落仅由氢营养菌组成，主要是甲烷菌属。正如qPCR在单室反应器中所展示的那样，由于存在阴极产生的氢，导致地球细菌和产甲烷菌的丰度增加。在两室反应器中，与一室反应器相比，产甲烷菌的丰度降低了98％。将氢气添加到两室反应器的阳极室中，可回收大量的产甲烷菌和土杆菌。产甲烷菌/细菌的比率通过使用辅助因子F确认₄₂₀产甲烷菌的自发荧光。讨论了反应器设置对开发初级微生物电化学技术的影响，理由是通常必须首选两室反应器。然而，它们的使用培养的生物膜阳极时，为表明产甲烷菌之间互养相互作用inheres折衷地杆菌减少，但pH值降低可能影响微生物过程。

更新日期：2020-07-06

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