A sensitive micellar electrokinetic chromatography method is presented to simultaneously quantify ofloxacin, gatifloxacin, dexamethasone sodium phosphate and prednisolone acetate. The method has the advantages of being rapid, accurate, reproducible, ecologically acceptable and sensitive. The electrophoretic separation utilized 20 mm borate buffer as background electrolyte with pH 10.0 ± 0.1 and 50 mm sodium dodecyl sulfate as a micelle forming molecule. A capillary tube (50 μm i.d., 33 cm) of fused silica was used and on‐column diode array detection at 243 nm for dexamethasone sodium phosphate and prednisolone acetate, and 290 nm for ofloxacin and gatifloxacin. Various factors were optimized such as the background electrolyte (type, concentration and pH), addition of sodium dodecyl sulfate and its concentration, detection wavelength, applied voltage and injection parameters. The studied drugs were efficiently separated in 6.2 min, at 20 kV with high resolution. The greenness of the method was estimated using an eco‐scale tool and the presented method was found to have excellent green characteristics. The method was validated in conformance with International Conference on Harmonization guidelines, with acceptable accuracy, precision and selectivity. The suggested method can be employed for the economic analysis of the four drugs in dissimilar binary combinations of eye drops saving solvents and chemicals.

中文翻译：

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绿色胶束电动色谱方法的逐步优化和灵敏度提高，可以同时测定各种二元眼科制剂中存在的某些氟喹诺酮类和糖皮质激素。

提出了一种灵敏的胶束电动色谱方法，可同时定量测定氧氟沙星，加替沙星，地塞米松磷酸钠和泼尼松龙的含量。该方法具有快速，准确，可重现，生态学上可接受和灵敏的优点。电泳分离使用20 m m硼酸盐缓冲液作为背景电解质，pH 10.0±0.1和50 m m十二烷基硫酸钠作为胶束形成分子。使用熔融石英毛细管（内径50μm，33 cm），地塞米松磷酸钠和泼尼松龙醋酸盐在243 nm处进行柱上二极管阵列检测，氧氟沙星和加替沙星在290 nm处进行柱上二极管阵列检测。优化了各种因素，例如背景电解质（类型，浓度和pH），十二烷基硫酸钠的添加及其浓度，检测波长，施加电压和进样参数。在20 kV的高分辨率下，在6.2分钟内有效分离了所研究的药物。使用生态规模工具估算了该方法的绿色度，发现该方法具有出色的绿色特性。该方法已按照国际协调会议指南进行了验证，准确性可接受，精度和选择性。所建议的方法可用于以节省溶剂和化学药品的不同滴眼液二元组合形式对四种药物进行经济分析。