Given that many people suffer from large‐area skin damage, skin regeneration is a matter of high concern. Here, an available method is developed for the formation of large‐area robust skins through three stages: fabrication of a biodegradable sealant‐loaded nanofiber scaffold (SNS), skin tissue reconstruction, and skin regeneration. First, a microfluidic blow‐spinning strategy is proposed to fabricate a large‐scale nanofiber scaffold with an area of 140 cm × 40 cm, composed of fibrinogen‐loaded polycaprolactone/silk fibroin (PCL/SF) ultrafine core–shell nanofibers with mean diameter of 65 nm. Then, the SNS forms, where the gelling reaction of fibrin sealant occurs in situ between thrombin and fibrinogen on PCL/SF nanofiber surface, to promote the migration and proliferation of fibroblasts, accelerating skin regeneration. Through an in vivo study, it is shown that the SNS can rapidly repair acute tissue damage such as vascular bleeding and hepatic hemorrhage, and also promote angiogenesis, large‐area abdominal wall defect repair, and wound tissue regeneration for medical problems in the world. Besides, it avoids the risk of immune rejection and secondary surgery in clinical applications. This strategy offers a facile route to regenerate large‐scale robust skin, which shows great potential in abdominal wall defect repair.

中文翻译：

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从可生物降解的填充密封剂的纳米纤维支架到微组织吹纺的大规模制造坚固的人造皮肤。

鉴于许多人遭受大面积皮肤损害，因此皮肤再生是一个备受关注的问题。在此，我们通过三个阶段开发了一种可用于形成大面积坚固皮肤的方法：制造可生物降解的填充有密封剂的纳米纤维支架（SNS），皮肤组织重建和皮肤再生。首先，提出了一种微流吹纺策略，以制造一个面积为140 cm×40 cm的大型纳米纤维支架，该支架由纤维蛋白原负载的聚己内酯/丝素蛋白（PCL / SF）超细核壳纳米纤维组成，平均直径65纳米。然后，形成SNS，其中PCL / SF纳米纤维表面上的凝血酶和纤维蛋白原之间原位发生血纤蛋白封闭剂的胶凝反应，以促进成纤维细胞的迁移和增殖，加速皮肤再生。通过体内研究，结果表明，SNS可以快速修复急性组织损伤，例如血管出血和肝出血，并且还可以促进血管生成，大面积腹壁缺损修复和伤口组织再生，从而解决世界上的医疗问题。此外，它避免了临床应用中免疫排斥和二次手术的风险。该策略提供了一种简便的途径来再生大规模坚固的皮肤，这在修复腹壁缺损方面显示出巨大的潜力。