Objective

To explore the composition characteristics of atmospheric fine particulate matter (PM2.5) and bronchoalveolar lavage fluid (BALF), and their impact on the development of chronic obstructive pulmonary disease (COPD).

Methods

The atmospheric PM2.5 samples and BALF samples from COPD patients were collected from June 2, 2017 to October 30, 2018, and allocated into a high-risk of PM2.5 inhalation group and a low-risk PM2.5 inhalation group according to the heating season in Harbin. Inorganic elements were detected by ICP-MS, and polycyclic aromatic hydrocarbons (PAHs) were detected by GC/MS.

Results

Twenty-six inorganic elements were found in 54 BALF specimens. There was a high correspondence in inorganic elements between BALF and atmospheric PM2.5. Trace elements Cr, Mn, V, and Co, and toxic trace elements Al, Pb, Cd, As, and Ag were above the upper limit of normal blood. There were significant higher K, Ti, Fe, Co, Cu, Se, Rb, Ag, and Sb in BALF of the high-risk PM2.5 inhalation group (p < 0.05). Sixteen PAHs were detected in 32 BALF samples. The main components of BALF and atmospheric PM2.5 were the high molecular weight PAHs, and the species and concentration of PAHs in BALF and atmospheric PM2.5 are highly consistent.

Conclusion

The types and concentrations of inorganic elements and PAHs in BALF of COPD patients are highly consistent with those of atmospheric PM2.5. The sustained high concentrations of Benzo(a)anthracene, Chrysene, Benzo(b)Fluoranthene, Benzo(k)Fluoranthene, Indeno(123-c,d)Pyrene, and Benzo(a)Pyrene in BALF of COPD patients may have long-term adverse effects on COPD patients.

中文翻译：

---

东北地区COPD患者大气细颗粒物和支气管肺泡灌洗液中无机元素和多环芳烃的季节性特征组成

目的

探讨大气细颗粒物（PM2.5）和支气管肺泡灌洗液（BALF）的组成特征，以及它们对慢性阻塞性肺疾病（COPD）发展的影响。

方法

从2017年6月2日至2018年10月30日收集来自COPD患者的大气PM2.5样本和BALF样本，并根据以下规定分为高风险PM2.5吸入组和低风险PM2.5吸入组。哈尔滨的采暖季节。ICP-MS检测无机元素，GC / MS检测多环芳烃（PAHs）。

结果

在54个BALF标本中发现了26种无机元素。BALF与大气PM2.5之间的无机元素高度对应。微量元素Cr，Mn，V和Co以及有毒微量元素Al，Pb，Cd，As和Ag均高于正常血液的上限。高风险PM2.5吸入组的BALF中有较高的K，​​Ti，Fe，Co，Cu，Se，Rb，Ag和Sb（p <0.05）。在32个BALF样品中检测到16种PAH。BALF和大气PM2.5的主要成分是高分子量PAH，而BALF和大气PM2.5中PAH的种类和浓度高度一致。

结论

COPD患者BALF中无机元素和PAHs的类型和浓度与大气PM2.5高度一致。在COPD患者的BALF中持续高浓度的苯并（a）蒽，苯，苯并（b）氟蒽，苯并（k）氟蒽，茚并（123- c，d）和苯并（a）have对COPD患者的长期不良影响。