Abstract Designing advanced biomaterials with regenerative and drug delivering functionalities remains a challenge in the field of tissue engineering. In this paper we present the design, development, and a use case of an electrospun nano-biocomposite scaffold composed of silk fibroin (SF), hardystonite (HT), and gentamicin (GEN). The fabricated SF nanofiber scaffolds provide mechanical support while HT acts as a bioactive and drug carrier, on which GEN is loaded as an antibacterial agent. Antibacterial zone of inhibition (ZOI) results indicate that the inclusion of 3–6 wt% GEN significantly improves the antibacterial performance of the scaffolds against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) bacteria, with an initial burst release of 10–20% and 72–85% total release over 7 days. The release rate of stimulatory silicon ions from SF-HT scaffolds reached 94.53±5 ppm after 7 days. Cell studies using osteoblasts show that the addition of HT significantly improved the cytocompatibility of the scaffolds. Angiogenesis, in vivo biocompatibility, tissue vascularization, and translatability of the scaffolds were studied via subcutaneous implantation in a rodent model over 4-weeks. When implanted subcutaneously, the GEN-loaded scaffold promoted angiogenesis and collagen formation, which suggests that the scaffold may be highly beneficial for further bone tissue engineering applications.

中文翻译：

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用于组织工程应用的丝素蛋白-硬石-庆大霉素纳米纤维支架的体外和体内评价

摘要 设计具有再生和药物输送功能的先进生物材料仍然是组织工程领域的一个挑战。在本文中，我们介绍了由丝素蛋白 (SF)、硬石 (HT) 和庆大霉素 (GEN) 组成的电纺纳米生物复合支架的设计、开发和用例。制造的 SF 纳米纤维支架提供机械支撑，而 HT 作为生物活性和药物载体，GEN 作为抗菌剂加载在其上。抗菌抑菌圈 (ZOI) 结果表明，包含 3-6 wt% GEN 显着提高了支架对革兰氏阴性大肠杆菌（E.coli）和革兰氏阳性金黄色葡萄球菌（S.aureus）细菌的抗菌性能, 7 天内的初始突释量为 10-20%，总释放量为 72-85%。SF-HT 支架中刺激性硅离子的释放率在 7 天后达到 94.53±5 ppm。使用成骨细胞的细胞研究表明，添加 HT 显着提高了支架的细胞相容性。血管生成、体内生物相容性、组织血管化和支架的可翻译性通过皮下植入啮齿动物模型进行了 4 周的研究。当皮下植入时，载有 GEN 的支架促进血管生成和胶原形成，这表明支架可能对进一步的骨组织工程应用非常有益。通过皮下植入啮齿动物模型超过 4 周，研究了组织血管化和支架的可平移性。当皮下植入时，载有 GEN 的支架促进血管生成和胶原形成，这表明支架可能对进一步的骨组织工程应用非常有益。通过皮下植入啮齿动物模型超过 4 周，研究了组织血管化和支架的可平移性。当皮下植入时，载有 GEN 的支架促进血管生成和胶原形成，这表明支架可能对进一步的骨组织工程应用非常有益。