Colletotrichum gloeosporioides is a main cause of rubber anthracnose, which results in very large losses for the natural rubber industry. In this study, an actinomycete strain gz-8 was isolated and had strong antagonistic activity against C. gloeosporioides, with an inhibition rate of 72.5 %. Strain gz-8 was identified as Streptomyces badius. Three active compounds were separated from S. badius gz-8 and identified as feigrisolide B, feigrisolide C and dinactin according to the mass spectrometry and NMR-spectra results. In the three compounds, dinactin exhibited the best antifungal activity against C. gloeosporioides, with an EC₅₀ value of 2.55 μg/mL, and its minimum inhibitory concentration was 44 μg/mL. Dinactin had broad inhibitory activities against nine other pathogenic fungi, and it also had an obvious control effect on rubber anthracnose comparable to that of chlorothalonil. Dinactin could inhibit the conidiogenesis and spore germination of C. gloeosporioides. This report will contribute to understanding the antifungal activity of dinactin against C. gloeosporioides.

炭疽菌是橡胶炭疽病的主要原因，这给天然橡胶工业造成很大损失。在该研究中，分离出了放线菌菌株gz-8 ，其对球孢梭菌具有很强的拮抗活性，抑制率为72.5％。gz-8菌株被鉴定为重链霉菌。三种活性化合物从分离S.栗褐GZ-8，并根据质谱和NMR谱的结果鉴定为feigrisolide B，feigrisolide C和dinactin。在这三种化合物中，dinactin表现出对C. gloeosporioides的最佳抗真菌活性，EC ₅₀值为2.55μg/ mL，最小抑菌浓度为44μg/ mL。Dinactin对其他9种病原真菌具有广泛的抑制活性，并且它对橡胶炭疽病的控制效果也与百菌清相当。Dinactin可以抑制C. gloeosporioides的分生和孢子萌发。该报告将有助于了解dinactin对球孢梭菌的抗真菌活性。