Biocompatible and antibacterial multi-layer coatings of hydroxyapatite (HA)-Ag/SiO₂/TiN/Ti were obtained on the Ti-6Al-4V alloy, by means of the magnetron sputtering technique. During characterization of the coatings, the chemical composition was evaluated by energy dispersive X-ray spectroscopy and the phase analysis was carried out by X-ray diffraction. The morphology of the coatings was observed by field emission scanning electron microscopy, while transmission electron microscopy was used to appreciate their structure. The adhesion of the coatings to the substrate was evaluated by micro scratch test. The in vitro biological response was evaluated in terms of cytotoxicity, adhesion and differentiation of mouse mesenchymal stem cells, as well as adhesion and bacterial viability of Staphylococcus aureus strain. Through the compositional study carried out, the deposition of the HA phase was verified, with a Ca/P ratio close to 1.67 and the characteristic diffraction peaks of this compound. The structural study of the coatings evidenced the obtention of multi-layer architectures. The use of an intermediate SiO₂/TiN/Ti trilayer was found to improve adhesion between HA-Ag and the substrate by 84%. Finally, the in vitro biological tests carried out indicated a potentially non-toxic character in the coatings. Additionally, an antibacterial effect was registered at low concentrations of Ag (<0.25 mg/L).

采用磁控溅射技术在Ti-6Al-4V合金上制备了生物相容性抗菌的羟基磷灰石(HA)-Ag/SiO ₂ /TiN/Ti多层涂层。在涂层表征过程中，通过能量色散 X 射线光谱评估化学成分，并通过 X 射线衍射进行物相分析。通过场发射扫描电子显微镜观察涂层的形貌，同时使用透射电子显微镜观察其结构。通过微划痕试验评价涂层对基材的附着力。从小鼠间充质干细胞的细胞毒性、粘附和分化，以及金黄色葡萄球菌菌株的粘附和细菌活力方面评价体外生物学反应。通过成分研究，验证了HA相的沉积，Ca/P比接近1.67，并具有该化合物的特征衍射峰。涂层的结构研究证明了多层结构的获得。研究发现，使用中间 SiO ₂ /TiN/Ti 三层可以将 HA-Ag 与基材之间的粘附力提高 84%。最后，进行的体外生物学测试表明涂层具有潜在的无毒特性。此外，低浓度 Ag (<0.25 mg/L) 也具有抗菌作用。