Single cell oil (SCO) represents an outstanding alternative to both fossil sources and vegetable oils from food crops waste. In this work, an innovative two-step process for the conversion of cellulosic paper mill waste into SCO was proposed and optimised. Hydrolysates containing glucose and xylose were produced by enzymatic hydrolysis of the untreated waste. Under the optimised reaction conditions (Cellic® CTec2 25 FPU/g glucan, 48 h, biomass loading 20 g/L), glucose and xylose yields of 95 mol% were reached. The undetoxified hydrolysate was adopted as substrate for a batch-mode fermentation by the oleaginous yeast Lipomyces starkeyi. Lipid yield, content for single cell, production and maximum oil productivity were 20.2 wt%, 37 wt%, 3.7 g/L and 2.0 g/L/d respectively. This new generation oil, obtained from a negative value industrial waste, represents a promising platform chemical for the production of biodiesel, biosurfactants, animal feed and biobased plastics.

单细胞油（SCO）代表了化石资源和粮食作物废料中的植物油的出色替代品。在这项工作中，提出并优化了将纤维素造纸厂废物转化为SCO的创新性两步过程。含有葡萄糖和木糖的水解产物是通过酶处理未处理的废物而产生的。在优化的反应条件下（Cellec®CTec2 25 FPU / g葡聚糖，48 h，生物量负载20 g / L），葡萄糖和木糖收率达到95 mol％。未脱氧的水解产物被用作产油酵母Lipomyces starkeyi分批发酵的底物。脂质产率，单细胞含量，产量和最大油生产率分别为20.2重量％，37重量％，3.7g / L和2.0g / L / d。从负值工业废物中获得的新一代石油，是生产生物柴油，生物表面活性剂，动物饲料和生物基塑料的有前途的平台化学品。