Global warming is a surging issue that has provoked the demand of green process to mitigate carbon dioxide. In this context, RuBisCo-equipped Escherichia coli has first developed and evaluated the CO₂-assimiliable capability based on the mass balance in three devices: Flask-based in CO₂ incubator (FIC), two-layered device (TLD) and CO₂ bubbling device (CBD) systematically. With the forced diffusion of 5% CO₂ in CBD, which confers an efficient attack of CO₂ to RuBisCo, the CO₂ assimilation increased from -5.03 to -2.63 g-CO₂/g-DCW. Furthermore, boosted CO₂ assimilation ability was observed by co-expression of GroELS chaperone with 71% reduction on CO₂ release. By DNA sequencing and tandem MS/MS analysis, the toxicity of RuBisCo and PRK was identified to interfere the sugar metabolism and energy producing, while the cell morphology was changed and observed in RuBisCo-equipped E. coli. Our study provides a new perspective of higher CO₂ assimilation for sustainable to eco-friendly green bioprocess.

全球变暖是一个激增的问题，已经激起了绿色工艺以减少二氧化碳的需求。在这种情况下，配备RuBisCo的大肠杆菌首先基于三种设备的质量平衡开发并评估了可与CO ₂结合的能力：基于烧瓶的CO ₂培养箱（FIC），两层设备（TLD）和CO ₂鼓泡装置（CBD）。随着CBD中5％CO ₂的强制扩散（赋予CO ₂对RuBisCo有效的攻击），CO ₂同化从-5.03增加到-2.63 g-CO ₂ / g-DCW。此外，提高了CO ₂通过共表达GroELS分子伴侣观察到同化能力，CO ₂释放降低71％。通过DNA测序和串联MS / MS分析，确定了RuBisCo和PRK的毒性可干扰糖的代谢和产生能量，同时在配备RuBisCo的大肠杆菌中改变并观察到细胞形态。我们的研究提供了更高的CO ₂同化率的新观点，以实现可持续的生态友好型绿色生物过程。