Manure from medicated livestock contains pharmaceutical antibiotics and antibiotic resistance genes (ARGs). Bioavailable antibiotics trigger further ARGs amplification during manure storage. It was tested whether biochar lowers the bioavailability of the antibiotics sulfamethazine (SMZ), ciprofloxacin (CIP), oxytetracycline (OTC) and florfenicol (FF) in manure and the amplification of sul1 and tet(W) ARGs. To that end, liquid pig manure was treated with 5% (w/w) pinecone biochar (BCP). Antibiotics dissipated during 30-d incubation in the order SMZ < OTC < CIP < FF. Added BCP further immobilized SMZ, OTC and CIP, while the effect was not significant for FF. Both sul1 and tet(W) ARGs copy numbers significantly increased by factors of 5.8 and 2.5, respectively, in OTC and SMZ spiked manure. The abundance of sul1 was significantly decreased in BCP amended manure, while the impact on tet(W) was less. Consequently, biochar is suitable for the management of antibiotics contaminated manure during storage.

含药牲畜的粪便包含药物抗生素和抗生素抗性基因（ARG）。生物可利用的抗生素在粪肥储存期间触发了进一步的ARGs扩增。测试了生物炭是否降低了粪便中抗生素磺胺二甲嘧啶（SMZ），环丙沙星（CIP），土霉素（OTC）和氟苯尼考（FF）的生物利用度以及sul 1和tet（W）ARGs的扩增。为此，用5％（w / w）的松果生物炭（BCP）处理猪粪液。孵育30天后，抗生素以SMZ <OTC <CIP <FF的顺序消散。加入BCP进一步固定了SMZ，OTC和CIP，但对FF效果不明显。无论SUL 1和TET（W）在OTC和SMZ加标肥料中，ARG的拷贝数分别显着增加了5.8和2.5倍。BCP改良粪肥中sul 1的丰度显着降低，而对tet（W）的影响较小。因此，生物炭适合于在存储过程中处理受污染的抗生素的抗生素。