This work aimed to study the behavior of antibiotic resistance genes (ARGs) in constructed wetlands with different exogenous inducers additions (oxytetracycline and its resistant bacteria) by high-throughput quantitative polymerase chain reaction. Results indicated that constructed wetlands have the potential to reduce ARGs relative abundances in wastewater, and the total ARGs removal efficiency could exceed 60%. ARGs profile in the effluent differed from that in the influent, and that did not directly reflect the export of dominant ARGs in wetland biofilms. Meanwhile, the highest levels of detected numbers and relative abundances of ARGs were 43 and 3.35×10^-1 for control system and 44 and 6.40×10^-1 for treatment system, respectively, which meant that ARGs generation in wetlands were inevitable, and antibiotic and antibiotic-resistant bacteria from wastewater could indeed promote ARGs abundance in the system. Compared to the single roles of inducers, their synergistic role had a more significant influence on ARGs relative abundance.

这项工作旨在通过高通量定量聚合酶链反应研究在人工湿地中添加不同外源性诱导物（土霉素及其耐药菌）的抗生素抗性基因（ARG）的行为。结果表明，人工湿地有可能减少废水中ARGs的相对丰度，并且ARGs的去除总效率可以超过60％。出水中的ARGs谱图与进水中的ARGs谱图不同，这并不能直接反映湿地生物膜中主要ARGs的出口。同时，对于控制系统，ARG的检测数量和相对丰度的最高水平分别为43和3.35×10 ^-1，而控制系统为44和6.40×10 ^-1对于处理系统而言，这意味着在湿地中产生ARGs是不可避免的，废水中的抗生素和抗药性细菌的确可以促进系统中ARGs的丰度。与单一的诱导物相比，它们的协同作用对ARGs的相对丰度有更大的影响。