Interfacing photosynthetic protein complexes with electrodes is frequently used for the identification of electron transfer mechanisms and the fabrication of biosensors. Binding of herbicide compounds to the terminal plastoquinone Q_B at photosystem II (PSII) causes disruption of electron flow that is associated with a diminished performance of the associated biodevice. Thus, the principle of electron transport inhibition at PSII can be used for herbicide detection and has inspired the fabrication of several biosensors for this purpose. However, the biosensor performance may reveal a more complex behavior than generally expected. As we present here for a photobioelectrode constituted by PSII embedded in a redox polymer matrix, the effect caused by inhibitors does not only impact the electron transfer from PSII but also the properties of the polymer film used for immobilization and electrical wiring of the protein complexes. Incorporation of phenolic inhibitors into the polymer film surprisingly translates into enhanced photocurrents and, in particular cases, in a higher stability of the overall electrode architecture. The achieved results stress the importance to evaluate first the possible influence of analytes of interest on the biosensor architecture as a whole and provide important insights for consideration in future design of bioelectrochemical devices.

光合蛋白复合物与电极的接口通常用于电子转移机制的识别和生物传感器的制造。除草剂化合物与末端质体醌Q _B的结合在光系统II（PSII）处的电子束会引起电子流的中断，这与相关生物设备的性能下降有关。因此，在PSII处抑制电子传输的原理可用于除草剂检测，并启发了为此目的制造几种生物传感器。但是，生物传感器的性能可能显示出比一般预期更复杂的行为。正如我们在这里展示的，由嵌入氧化还原聚合物基质中的PSII构成的光生物电极，抑制剂引起的效应不仅影响PSII的电子转移，而且还影响用于固定和蛋白质复合物电连接的聚合物膜的性能。在聚合物薄膜中掺入酚类抑制剂会出人意料地转化为增强的光电流，在某些情况下，整体电极架构的稳定性更高。所获得的结果强调了首先评估目标分析物对整个生物传感器结构的可能影响的重要性，并为将来在生物电化学设备的设计中考虑提供了重要的见识。