Abstract

The lux biosensor of E. coli MG1655 (pDinI::lux) was constructed and a comparative study of the SOS response of three biosensors E. coli MG1655 (pRecA::lux), E. coli MG1655 (pColD::lux), and E. coli MG1655 (pDinI::lux) under the action of genotoxic agents was performed. The listed biosensors were named, respectively, PRecA, PColD, and PDinI. The response amplitude (RA) was chosen as an indicator of the SOS response level of lux biosensors. It was shown that RA of the PDinI biosensor was more expressed than RA of the PRecA biosensor under the action of hydrogen peroxide, alkylating agents such as NMU, MMS, and streptozotocin, antibacterial agent such as dioxidine, and cytostatics such as mitomycin C and cisplatin. Antimetabolite 5-fluorouracil showed activity only with PDinI. Furacilin and 4-NQO, whose metabolites form adducts with DNA, were more active on PColD than on PRecA and PDinI. DNA gyrase inhibitors such as nalidixic acid and ciprofloxacin were less active on PDinI than on PColD and PRecA. Overall, among 13 tested substances, 8 more actively induced SOS response in the PDinI biosensor than in PColD and PRecA. At the same time, 5-fluororacil induced SOS response only with the PDinI biosensor. It was concluded that the PDinI biosensor can be successfully used for the primary detection of potential genotoxicants by their ability to induce SOS response in E. coli cells.

中文翻译：

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包含pRecA :: lux，pColD :: lux和pDinI :: lux质粒的大肠杆菌特异性Lux生物传感器，用于检测遗传毒性剂

摘要

构建了大肠杆菌MG1655（pDinI :: lux）的lux生物传感器，并对三种生物传感器大肠杆菌MG1655（pRecA :: lux），大肠杆菌MG1655（pColD :: lux）和SOS响应进行了比较研究。在遗传毒性剂的作用下进行了大肠杆菌MG1655（pDinI :: lux）。列出的生物传感器分别命名为PRecA，PColD和PDinI。选择响应幅度（RA）作为lux生物传感器的SOS响应水平的指标。结果表明，PDinI生物传感器的RA在过氧化氢，烷化剂（如NMU，MMS和链脲佐菌素），抗菌剂（如双氧精）和细胞抑制剂（如丝裂霉素C和顺式）的作用下比PRecA生物传感器的RA表达更高。铂金。抗代谢物5-氟尿嘧啶仅对PDinI具有活性。富拉西林和4-NQO的代谢产物与DNA形成加合物，对PColD的活性高于对PRecA和PDinI的活性。DNA促旋酶抑制剂（如萘啶酸和环丙沙星）对PDinI的活性低于对PColD和PRecA的活性。总体而言，在13种被测物质中，PDinI生物传感器中8种比PColD和PRecA更能积极诱导SOS反应。同时，仅使用PDinI生物传感器，5-氟尿嘧啶就能诱导SOS反应。结论是，PDinI生物传感器通过在大肠杆菌细胞中诱导SOS应答的能力，可以成功地用于潜在遗传毒性的初步检测。