Electroacupuncture (EA) can improve myocardial ischemia (MI) injury; nevertheless, the mechanism is not entirely clear. And there were disagreements about whether the effect of EA at acupoint in disease-affected meridian is better than EA at acupoint in non-affected meridian and sham acupoint. Here, we showed that the effect of EA at Neiguan (PC6) is better than EA at Hegu (LI4) and sham acupoint in affecting RPP and ECG, increasing ATP and ADO production, decreasing AMP production, and upregulating the mRNA expression levels of A1AR, A2aAR, and A2bAR; knockdown of A1AR or A2bAR reversed the effect of EA at PC6 in alleviating MI injury; knockdown of A2aAR had no influence on the cardiac protection of EA at PC6; thus, the cardioprotective effect of EA at PC6 needs A1AR and A2bAR, instead of A2aAR; considering that the cardio protection of adenosine receptor needs activation of other adenosine receptors, one of the reasons may be that after silence of A1AR or A2bAR, EA at PC6 could not impact the expression levels of the other two adenosine receptors, and after silence of A2aAR, EA at PC6 could impact the expression levels of A1AR and A2bAR. These results suggested that EA at PC6 may be a potential and effective treatment for MI by activation of A1AR and A2bAR.

电针（EA）可改善心肌缺血（MI）损伤；然而，机制并不完全清楚。电针在病经穴位的作用是否优于非病经穴位和假穴位，存在不同意见。在这里，我们表明电针在内关（PC6）的作用优于合谷（LI4）和假穴位的电针在影响RPP和ECG、增加ATP和ADO产生、减少AMP产生以及上调A1AR的mRNA表达水平方面的作用。 、A2aAR 和 A2bAR；A1AR 或 A2bAR 的敲低逆转了 EA 在 PC6 上减轻 MI 损伤的作用；A2aAR 的敲低对 EA 在 PC6 的心脏保护没有影响；因此，EA 对 PC6 的心脏保护作用需要 A1AR 和 A2bAR，而不是 A2aAR；考虑到腺苷受体的心脏保护需要激活其他腺苷受体，原因之一可能是A1AR或A2bAR沉默后，PC6的EA不能影响其他两种腺苷受体的表达水平，而A2aAR沉默后, PC6 的 EA 可能影响 A1AR 和 A2bAR 的表达水平。这些结果表明，PC6 的 EA 可能是通过激活 A1AR 和 A2bAR 治疗 MI 的潜在有效治疗方法。