Bacterial reaction centers (BRC) from Rhodobacter sphaeroides were found to accelerate, about 100-fold, the reaction between tetryl (2,4,6-trinitrophenylmethylnitramine) explosive and n-lauryl-N-N-dimethylamine-N-oxide (LDAO) that results in the formation of picric acid-like product with characteristic UV–VIS absorption spectrum with peaks at 345 and 415 nm. Moreover, this product also affects the spectra of BRC cofactors in the NIR spectral region and stabilizes the conformational changes associated with slow charge recombination. The evolution of the NIR absorption changes correlated with the kinetics of the product formation. Comparison between the wild-type and the R26 carotenoid-less strain indicates that tetryl-LDAO reaction is roughly five times faster for R26, which allows for identifying the carotenoid binding site as the optimal reaction site. Another, less-defined reaction site is located in the BRC’s hydrophobic cavity. These effects are highly selective for tetryl and not observed for several other widespread nitric explosives; slowed-down charge recombination allows for distinguishing between tetryl and Q_B-site herbicides. The current limit of detection is in the ppb range or ~ 100 nM. Details of the molecular mechanisms of the reactions and perspectives of using these effects in bioassays or biosensors for explosives detection are also discussed.

从细菌反应中心（BRC）球形红细菌被发现加速，约100倍，特屈儿（2,4,6- trinitrophenylmethylnitramine）炸药和之间的反应Ñ -lauryl- ñ - ñ -dimethylamine- Ñ氧化物（LDAO）导致形成苦味酸状产物，具有特征性的UV-VIS吸收光谱，并在345和415 nm处出现峰。此外，该产物还影响近红外光谱区域中BRC辅助因子的光谱，并稳定与缓慢电荷重组相关的构象变化。NIR吸收变化的演变与产物形成的动力学相关。野生型菌株和不含R26的类胡萝卜素菌株之间的比较表明，对于R26，tetryl-LDAO反应的速度大约快五倍，这可以将类胡萝卜素结合位点确定为最佳反应位点。另一个不太明确的反应位点位于BRC的疏水腔中。这些效应对替替勒具有高度选择性，而其他几种广泛的硝酸炸药则未观察到这些效应。_B位除草剂。检测的电流极限在ppb范围内或〜100 nM。还讨论了反应的分子机理的详细信息以及在生物测定法或生物传感器中用于爆炸物检测的这些作用的观点。