Green algae have great potential for removing inorganic nutrients from anaerobic digestion effluent (ADE), but there is insufficient available magnesium (Mg) in the ADE from aquatic macrophytes for effective algal growth. In this study, we determined suitable pH and hydraulic retention time (HRT) for maximizing growth of the alga Chlorella sorokiniana and nutrient removal efficiency with a flow-through continuous cultivation system. This used ADE from aquatic macrophytes, adjusted to pH 5.0, 6.0, 6.5 and 7.0 at two HRTs of 6 and 8 days. The highest C. sorokiniana biomass concentration was obtained in the pH 6.5 treatment, being 0.50 and 0.67 g L⁻¹ at 6 and 8 days of HRT, respectively, without Mg enrichment. Both were equivalent to 83.6 mg L⁻¹ day⁻¹ of biomass productivity. Removal rates of both ammonium nitrogen and phosphate phosphorus were almost 100% in pH 6.5 and 7.0 treatments and declined to below 60% in pH 5.0 and 6.0 treatments during the study period. The results demonstrated that a source medium pH of 6.5 using a continuous cultivation system (irrespective of the two HRTs), and increasing available Mg, was suitable for maximizing C. sorokiniana biomass productivity and nutrient removal from the ADE.

绿藻具有从厌氧消化液（ADE）去除无机营养的巨大潜力，但是ADE中有效水生植物中有效镁的含量不足，藻类无法有效生长。在这项研究中，我们确定了合适的pH值和水力停留时间（HRT），以通过流通式连续培养系统最大化藻类小球藻的生长和营养去除效率。这使用了水生植物的ADE，在6天和8天的两个HRT时将其pH值调节为5.0、6.0、6.5和7.0。在pH 6.5处理中获得了最高的梭状芽孢杆菌生物量浓度，在不进行Mg富集的情况下，HRT在第6天和第8天分别为0.50和0.67 g L ^-1。两者均相当于83.6 mg L ^-1生物量生产力的第^-1天。在研究期间，在pH 6.5和7.0处理中，铵态氮和磷酸盐磷的去除率几乎为100％，而在pH 5.0和6.0处理中，去除率降至60％以下。结果表明，使用连续培养系统（与两个HRT无关）的pH值为6.5的源培养基，并增加了可利用的Mg，适用于最大化梭菌衣原体的生物量生产力和从ADE去除营养。