Change in the climatic conditions due to uncontrolled emission of carbon dioxide is continuously encouraging to search alternative renewable energy sources. Corn stover is one of the several feedstocks being considered as growth medium for oil producing bacteria. India ranks third in the production of corn in the world. The present study focused on biodiesel synthesis from microbial oil, chemical profiling and evaluation of physicochemical properties of produced biodiesel. The bacterium was isolated from food waste resources and identified as Bacillus amyloliquefaciens (MF510169) on the basis of morphological‚ biochemical characters and 16S rRNA sequencing. The bacterium B. amyloliquefaciens (MF510169) was grown on corn stover as an ideal agro-waste feedstock. The bacterium grown on corn stover peels revealed maximum lipid production after acid hydrolysis (0.25% HCl) over other feedstocks used under study. The bacterium produces 3.8% oil under operating parameters, i.e. inoculum size, density, carbon and nitrogen source, C/N ratio, temperature, pH, rotational speed and fermentation period. The oil was transesterified using sulphuric acid (0.12%) as a catalyst to form fatty acids methyl esters. Transesterification reaction was optimised at various parameters like catalyst type, catalyst loading, oil: methanol ratio, temperature and reaction time. The chemical profiling of FAMEs revealed hexadecanoic acid methyl ester, 9-1, 2 octadecanoic acid methyl ester, octadecanoic acid methyl ester and methyl stearate as characterised by using GC–MS, GC-FID, FTIR and NMR techniques. The physicochemical properties of transesterified biodiesel revealed 85% similarity with the ASTM International Standards. The produced biodiesel from Bacillus could be exploited at commercial level after successful engine trial tests.

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利用农业废料从非食用油中合成生物柴油及其理化性质的评估

由于二氧化碳不受控制的排放而导致的气候条件变化不断鼓励寻找替代可再生能源。玉米秸秆是被认为是产油细菌生长培养基的几种原料之一。印度在世界玉米产量中排名第三。本研究的重点是从微生物油中合成生物柴油，化学分析以及所生产生物柴油的理化特性评估。从食物垃圾资源中分离出该细菌，并根据形态，生化特征和16S rRNA测序将其鉴定为解淀粉芽孢杆菌（MF510169）。细菌解淀粉芽孢杆菌（MF510169）是在玉米秸秆上生长的理想农作物原料。玉米秸秆皮上生长的细菌比其他研究原料在酸水解（0.25％HCl）后显示出最大的脂质产生。细菌在操作参数下产生3.8％的油，即接种物大小，密度，碳和氮源，C / N比，温度，pH，旋转速度和发酵时间。用硫酸（0.12％）作为催化剂使油酯交换，形成脂肪酸甲酯。在各种参数（例如催化剂类型，催化剂负载量，油：甲醇比，温度和反应时间）下优化了酯交换反应。FAME的化学分析显示十六烷酸甲酯，9-1、2十八烷酸甲酯，用GC-MS，GC-FID，FTIR和NMR技术表征十八烷酸甲酯和硬脂酸甲酯。酯交换生物柴油的理化特性显示与ASTM国际标准具有85％的相似性。从生产的生物柴油成功进行发动机试验后，可以在商业水平上利用芽孢杆菌。