Abstract

Cultured hydrocarbon-oxidizing bacteria have been isolated for the first time from bacterioplankton in urban Lake Beloye (Moscow). The taxonomic positions of two bacterial strains (2012B and 2012C) isolated from this lake have been determined. Lipids of the strain 2012B comprise C_14:0–C_19:0 fatty acids, the most abundant of them being C_15:0 (54%), C_16:0 (17%), C_17:0 (10%), and 10-methyl С_18:0 (3.5%). Lipids of the strain 2012C comprise C_14:0–C_19:0 fatty acids, the most abundant of them being C1_5:0 (45%), C_16:0 (32%), and C_17:0 (9%). A phylogenetic analysis of strain 2012B is performed using the nucleotide sequences of the 16S rRNA (KP779654.1) and alkB (KR422620.1) genes and the strain is identified as a typical member of the genus Rhodococcus spp. (Actinobacteria, Nocardiaceae). The combination of molecular identification and analysis of biochemical and physiological properties makes it possible to identify strain 2012B as Rhodococcusqingshengii 2012B. A phylogenetic analysis of strain 2012C is performed using the nucleotide sequences of 16S rRNA (MG966152) and shows the highest identity (99.57%) of strain 2012С with Pseudomonas psychrotolerans and Pseudomonas oryzihabitans

中文翻译：

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城市Beloye（莫斯科）的烃类氧化细菌：鉴定和系统发育分析

摘要

首次从城市贝洛伊湖（莫斯科）的浮游细菌中分离出培养的碳氢化合物氧化细菌。已经确定了从该湖中分离出的两种细菌菌株（2012B和2012C）的分类位置。菌株2012B的脂质包含C _14：0 –C _19：0脂肪酸，其中最丰富的是C _15：0（54％），C _16：0（17％），C _17：0（10％） ，和10-甲基С18 _：0（3.5％）。菌株2012C的脂质包含C _14：0 –C _19：0脂肪酸，其中最丰富的是C1 _5：0（45％），C _16：0（32％）和C _17：0（9％）。使用16S rRNA（KP779654.1）和alkB（KR422620.1）基因的核苷酸序列进行菌株2012B的系统发育分析，该菌株被鉴定为红球菌属的典型成员。（放线菌，诺卡氏菌）。分子鉴定和生化及生理特性分析的结合使得鉴定菌株2012B成为清红球菌2012B成为可能。使用16S rRNA（MG966152）的核苷酸序列进行了菌株2012C的系统发育分析，显示该菌株2012С与假单胞菌和米假单胞菌的同一性最高（99.57％）