Chlorpyrifos (CPF) is one of the most abundant and widely used pesticides in the world. CPF has detrimental effects on brain tissue, so it is possible to generate some neurodegenerative diseases. The aim of this study was to evaluate the effect of CPF on inducing the Parkinson’s disease affecting on central nervous system. 6 to 8-week-old animals were categorized into three groups. The first group was normal control which the animals did not received any treatment, while in the second group, CPF were injected (CPF; 5 mg/kg BW for 30 days intraperitoneally) and the sham group as the third group received DMSO. At the end of the CPF treatment, malondialdehyde (MDA) and superoxide dismutase (SOD) levels were measured in the brain tissues of rats. Proportion of neurons was analyzed by crystal violet assays and tunnel assay to detect apoptotic cells. Finally, the expression of GFAP and TH was investigated in the brain of animals. The results witnessed an increase in MDA and a decrease in SOD (P < 0.05) after the CPF treating. Moreover, results indicated that the proportion of neurons decreased in the second group vs. normal and sham groups significantly (P < 0.001). Additionally, in substantia nigra, the expression of GFAP had a significant increase and the TH had a remarkable decrease in CPF injected group in comparison to two other groups (P < 0.001). Furthermore, the numbers of apoptosis cells reduced in substantia nigra (P < 0.001) after the 30-day period of CPF injections. These results demonstrated that repeated exposure to CPF can induce PD via apoptotic cell death, histopathological disruption. It also altered the expression of dopaminergic neuron and changes the levels of oxidant and antioxidant enzymes in substantia nigra region which triggers PD. Hence, the CPF can be introduced as a risk factor for PD.

毒死rif（CPF）是世界上使用最广泛的农药之一。CPF对脑组织具有有害作用，因此可能会产生一些神经退行性疾病。这项研究的目的是评估CPF诱导帕金森氏病对中枢神经系统的影响。将6至8周大的动物分为三组。第一组是正常对照组，动物未接受任何治疗，而第二组，注射了CPF（CPF； 5 mg / kg体重，腹膜内注射30天），假手术组作为第三组接受了DMSO。CPF治疗结束时，在大鼠的脑组织中测出了丙二醛（MDA）和超氧化物歧化酶（SOD）的水平。通过结晶紫测定法和隧道测定法分析神经元的比例以检测凋亡细胞。最后，研究了动物大脑中GFAP和TH的表达。结果见证了MDA的增加和SOD的减少（C  <0.05）。此外，结果表明第二组神经元的比例明显低于正常组和假组（P  <0.001）。此外，与另外两个组相比，在CPF注射组中黑质中GFAP的表达显着增加，TH显着降低（P  <0.001）。此外，黑质（P CPF注射30天后<0.001）。这些结果表明，反复接触CPF可以通过凋亡细胞死亡，组织病理学破坏来诱导PD。它也改变了多巴胺能神经元的表达，并改变了黑质区域触发PD的氧化和抗氧化酶的水平。因此，可以将CPF作为PD的危险因素。