Insertion sequences (IS) are ubiquitous transposable elements with a very simple organization: two inverted repeats flanking a transposase coding gene. IS982 is one of 26 insertion sequence families known so far. With 70 registered members in the ISFinder database, this family remains somewhat unexplored, despite the association of many of its members with important features such as antibiotic resistance. IS982 has a fairly simple organization with a mean length of ca. 1 Kb, two inverted repeats with conserved 5′ AC 3′ ends flanking a transposase coding gene and direct repeats of variable lengths. Its transposase has a RNAse-H like chemistry with an atypical DDE motif. In this study, we first highlight the current knowledge on the IS982 family by dissecting its registered members and their characteristics. Secondly, we bring new insights into this old, yet uncharted IS family, by exploring its registered elements, as well as the genomic and proteomic databases of bacterial and archaeal strains. This probing showed that the presence and distribution of this family goes far beyond the clear-cut registry of ISFinder database.

中文翻译：

---

IS982 和亲属：对古老 IS 家族的新见解。


插入序列（IS）是普遍存在的转座元件，其组织非常简单：转座酶编码基因两侧的两个反向重复序列。 IS982 是迄今为止已知的 26 个插入序列家族之一。 ISFinder 数据库中有 70 名注册成员，尽管该家族的许多成员与抗生素耐药性等重要特征有关，但该家族在某种程度上仍未被探索。 IS982 有一个相当简单的组织，平均长度为 ca。 1 Kb，两个反向重复序列，具有位于转座酶编码基因侧翼的保守 5' AC 3' 末端和可变长度的正向重复序列。其转座酶具有类似 RNAse-H 的化学性质，具有非典型的 DDE 基序。在本研究中，我们首先通过剖析 IS982 家族的注册成员及其特征来强调当前对 IS982 家族的了解。其次，我们通过探索其注册元件以及细菌和古菌菌株的基因组和蛋白质组数据库，对这个古老但未知的 IS 家族带来了新的见解。此次探测表明，该家族的存在和分布远远超出了ISFinder数据库明确登记的范围。