The diamondback moth (Plutella xylostella, DBM) is an important pest of cruciferous vegetables. The use of chlorantraniliprole has been essential in the management of the DBM. However, in many countries and areas, DBM has become highly resistant to chlorantraniliprole. Three different DBM strains, susceptible (S), chlorantraniliprole-selected (R_c), and field-collected (R_b) resistant strains/populations were studied for the role of phenoloxidase in resistance development to the insecticide. By assaying the activity of phenoloxidase (PO) in the three different DBM strains, the results showed that the PO activity in the R_c strain was increased significantly compared with the S strain. The synergistic effects of quercetin showed that the resistant ratio (RR) of the QR_c larvae to chlorantraniliprole was decreased from 423.95 to 316.42-fold compared with the R_c larvae. Further studies demonstrated that the transcriptional and translational expression levels of PxPPO1 (P. xylostella prophenoloxidase-1 gene) and PxPPO2 (P. xylostella prophenoloxidase-2 gene) were increased to varying degrees compared with the S strain, such as the transcriptional expression levels of PxPPO2 were 24.02-fold that of the S strain. The responses of phenoloxidase were significantly different in chlorantraniliprole-resistant DBM.

中文翻译：

---

小菜蛾对小菜蛾对氯霉素的抗性中酚氧化酶的响应增加。（鳞翅目：P科）

小菜蛾（Plutella xylostella， DBM）是十字花科蔬菜的重要害虫。氯氰菊酯的使用对于DBM的管理至关重要。但是，在许多国家和地区，DBM均已对氯吡喹具有高度抗性。研究了三种不同的DBM菌株，即易感菌株（S），绿藻腈选择菌株（R _c）和田间采集菌株（R _b）的抗性菌株/种群对酚氧化酶在对杀虫剂产生抗性中的作用。通过分析三种不同DBM菌株中酚氧化酶（PO）的活性，结果表明R _c中的PO活性与S菌株相比，该菌株显着增加。槲皮素的协同效应表明，QR的抗性比（RR）_Ç幼虫氯虫苯甲酰胺，从423.95降低至316.42倍与R进行比较_Ç幼虫。证明的转录和翻译的表达水平进一步研究PxPPO1（小菜蛾酶原-1基因）和PxPPO2（小菜蛾酶原-2基因）的不同程度升高带有S菌株相比，如转录的表达水平PxPPO2是S菌株的24.02倍。耐氯霉素的DBM中酚氧化酶的反应显着不同。