BACKGROUND AND AIMS The twig cross-sectional area and the surface area of leaves borne on it are expected to be isometrically correlated across species (Corner's rules). However, how stable this relationship remains in time is not known. We studied the interspecific and intraspecific twig leaf area-cross-sectional area (la-cs) and other scaling relationships, including the leaf-shoot mass (lm-sm) scaling relationship, across a complete growing season. We also examined the influence of plant height, deciduousness and the inclusion of reproductive buds in the stability of the scaling relationships, and we discuss results from a functional perspective. METHODS We collected weekly current-year twigs of 6 Patagonian woody species that differed in growth form and foliar habit. We also used Embothrium coccineum's (Proteaceae) prominent inflorescences to assess whether or not reproductive buds alter the la-cs isometric relationship. Mixed effects models were fitted to obtain parameter estimates and to test whether interaction terms were nonsignificant (invariant) for the scaling relationships. KEY RESULTS The slope of the la-cs scaling relationship remained invariant across the growing season. Two species showed contrasting and disproportional (allometric) la-cs scaling relationships (slope ≠ 1). Scaling relationships significantly varied across growth form and foliar habit. The lm-sm scaling relationship differed between reproductive- and vegetative-origin twigs in E. coccineum, which was explained by a significantly lower leaf mass per area in the former. CONCLUSIONS Although phenology during the growing season appeared not to change leaf-shoot scaling relationships across species, we show that scaling relationships departed from the general trend of isometry as a result of within-species variation, growth form, foliar habit and the type of twig. The identification of these functional factors helps to understand variation in the general trend of Corner's rules.

中文翻译：

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Corner 规则通过了时间的考验：物候对叶-芽和其他尺度关系的影响很小

背景和目标 树枝的横截面积和其上的叶子的表面积预计在不同物种之间是等距相关的（Corner 规则）。然而，这种关系在时间上的稳定性如何尚不清楚。我们研究了整个生长季节的种间和种内枝叶面积-横截面积 (la-cs) 和其他比例关系，包括叶-芽质量 (lm-sm) 比例关系。我们还研究了植物高度、落叶性和包含生殖芽对缩放关系稳定性的影响，并从功能角度讨论了结果。方法我们每周收集 6 种巴塔哥尼亚木本树种的当年枝条，这些树种的生长形式和叶面习性各不相同。我们还使用了 Embothrium coccineum' s (Proteaceae) 突出的花序，以评估生殖芽是否改变 la-cs 等距关系。拟合混合效应模型以获得参数估计值并测试交互项对于标度关系是否不显着（不变）。主要结果 la-cs 比例关系的斜率在整个生长季节保持不变。两个物种表现出对比和不成比例的（异速生长）la-cs 比例关系（斜率 ≠ 1）。尺度关系因生长形式和叶面习性而异。E. coccineum 中生殖和营养来源的枝条之间的 lm-sm 比例关系不同，这可以解释为前者单位面积的叶质量显着降低。结论虽然生长季节的物候学似乎不会改变物种间的叶-芽比例关系，但我们表明，由于种内变异、生长形式、叶习性和树枝类型，比例关系偏离了等距的一般趋势。 . 识别这些功能因素有助于理解 Corner 规则总体趋势的变化。