Large-Area Aligned Fullerene Nanocrystal Scaffolds as Culture Substrates for Enhancing Mesenchymal Stem Cell Self-Renewal and Multipotency,ACS Applied Nano Materials

当前位置： X-MOL 学术 › ACS Appl. Nano Mater. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Large-Area Aligned Fullerene Nanocrystal Scaffolds as Culture Substrates for Enhancing Mesenchymal Stem Cell Self-Renewal and Multipotency
ACS Applied Nano Materials ( IF 5.3 ) Pub Date : 2020-07-04 , DOI: 10.1021/acsanm.0c00973
Jingwen Song ₁ , Xiaofang Jia ₂ , Kosuke Minami _{2,

3,

4} , Jonathan P. Hill ₂ , Jun Nakanishi ₂ , Lok Kumar Shrestha ₂ , Katsuhiko Ariga _{1,

2}

Affiliation

Development of human mesenchymal stem cell (hMSC)-based therapies is hindered by the limited availability of adult stem cells. Long-term retention of multipotency and stem-like phenotypes remains a key challenge in the large-scale in vitro expansion process. In this paper, we report the primary example of the use of highly aligned fullerene nanowhisker nanopatterned scaffolds to regulate precisely adhesion and function of hMSCs. Continuously tunable alignment of fullerene nanowhiskers was attained using a simple Langmuir–Blodgett approach. We have found that long-term multipotency retention and regenerative capacity are enhanced in cells cultured on the highly aligned fullerene nanowhisker nanopatterned surfaces mediated by the appropriate cell contractility and nucleus localization of Yes-associated protein. This study highlights the importance of the aligned fullerene nanowhisker nanopatterned surface as an attractive platform for stem cell expansion in vitro and for tissue engineering.

中文翻译：

大面积对齐的富勒烯纳米晶体支架作为文化基质增强间充质干细胞的自我更新和多能性。

成人间充质干细胞的有限可用性阻碍了基于人类间充质干细胞（hMSC）疗法的发展。长期保留多能性和茎样表型仍然是大规模体外试验中的关键挑战扩展过程。在本文中，我们报道了使用高度对齐的富勒烯纳米晶须纳米图案化支架来精确调节hMSCs粘附和功能的主要例子。使用简单的Langmuir-Blodgett方法可以实现富勒烯纳米晶须的连续可调排列。我们已经发现，在由适当的细胞收缩性和Yes相关蛋白的核定位介导的高度对齐的富勒烯纳米晶须纳米图案表面上培养的细胞中，长期多能性保留和再生能力得到增强。这项研究强调了对齐的富勒烯纳米晶须纳米图案表面作为体外扩增干细胞和组织工程的有吸引力的平台的重要性。

更新日期：2020-07-24

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11