Abstract

Context

Recent surveys have shown an association between proprotein convertase subtilisin/kexin type 9 (PCSK9) and oxidative stress.

Objective

In this investigation, the effect of evolocumab an anti-PCSK9 antibody was assessed against oxidative damage caused by hydrogen peroxide (H₂O₂) in human umbilical vein endothelial cells (HUVEC).

Material and methods

Viability of HUVEC was measured by MTT assay. Hydroperoxides and malondialdehyde (MDA) levels, and ferric reducing antioxidant power (FRAP) were detected in HUVEC that pre-treated with evolocumab and, then exposed to H₂O₂.

Results

Evolocumab significantly prevented the cytotoxicity induced by H₂O₂ at the concentrations of 5–100 µg/ml. Pre-treatment of HUVEC with evolocumab reduced hydroperoxides and MDA levels and also increased FRAP value in intra- and extra-cellular mediums compared with H₂O₂ stimulated cells at different concentration ranges.

Conclusion

This study displayed anti-oxidative and cytoprotective activities of evolocumab against oxidative damage caused by H₂O₂ in endothelial cells.

中文翻译：

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Evolocumab 是一种 PCSK9 抑制剂，可保护人类内皮细胞免受 H2O2 诱导的氧化应激

摘要

语境

最近的调查显示前蛋白转化酶枯草杆菌蛋白酶/kexin 9 型 (PCSK9) 与氧化应激之间存在关联。

客观的

在这项研究中，评估了 evolocumab 抗 PCSK9 抗体对人脐静脉内皮细胞 (HUVEC) 中过氧化氢 (H ₂ O _{2 ) 引起的氧化损伤的作用。}

材料与方法

HUVEC的活力通过MTT测定法测量。在用 evolocumab 预处理然后暴露于 H ₂ O ₂的 HUVEC 中检测到氢过氧化物和丙二醛 (MDA) 水平以及降低铁的抗氧化能力 (FRAP) 。

结果

Evolocumab在 5–100 µg/ml 的浓度下显着阻止了 H ₂ O ₂诱导的细胞毒性。与不同浓度范围的 H ₂ O ₂刺激细胞相比，用 evolocumab 预处理 HUVEC 降低了氢过氧化物和 MDA 水平，并且还增加了细胞内和细胞外培养基中的 FRAP 值。

结论

这项研究显示了 evolocumab 对内皮细胞中H ₂ O ₂引起的氧化损伤的抗氧化和细胞保护活性。