Abstract

Nannochloropsis oculata is a promising source of biodiesel due to the production of cellular lipid which can be converted to biodiesel through transesterification reaction. In this study, N. oculata cells were cultured under a medium light intensity and high CO₂ concentrations to improve cell growth and cellular lipid content. With an appropriate initial cell concentration, a 1.5-fold increase in cell number and a 4-fold increase in lipid content were achieved when N. oculata was cultured at a medium light intensity of 300 µEm⁻²s⁻¹ and 10-15% CO₂ compared to cells cultured at a low light intensity of 30 µEm⁻²s⁻¹ and 0.037% CO₂. The cultivation of N. oculata was further scaled up in a photobioreactor. When cultured with medium light intensity and high CO₂ concentration, N. oculata can obtain higher cell concentrations and lipid amounts. The microalgal biodiesel analyzed by gas chromatography revealed that high yield was achieved by culturing N. oculata under the combination of medium light illumination and high CO₂ concentration. When cultured in a scaled-up photobioreactor under stressful illumination and high CO₂ concentrations, N. oculata not only showed improved cell concentrations and lipid content, but also showed increased CO₂ depletion compared to cells cultured under low light and low CO₂.

摘要

Nannochloropsis oculata是一种很有前途的生物柴油来源，因为它产生的细胞脂质可以通过酯交换反应转化为生物柴油。在这项研究中，N. oculata细胞在中等光强度和高 CO ₂浓度下培养，以改善细胞生长和细胞脂质含量。在适当的初始细胞浓度下，当N. oculata在 300 µEm ^-2 s ^-1和 10-15%的中等光强度下培养时，细胞数量增加了 1.5 倍，脂质含量增加了 4 倍CO _{2与在 30 µEm} ^-2 s ^-1的低光强度下培养的细胞相比和 0.037% CO ₂。N. oculata的培养在光生物反应器中进一步扩大。在中等光强度和高CO ₂浓度下培养时，N. oculata可以获得更高的细胞浓度和脂质量。通过气相色谱分析的微藻生物柴油表明，在中等光照和高CO ₂浓度的组合下培养N. oculata获得了高产率。_{当在压力光照和高 CO 2}浓度下在放大的光生物反应器中培养时， N. oculata不仅表现出改善的细胞浓度和脂质含量，而且表现出增加的 CO₂消耗与在低光和低 CO ₂下培养的细胞相比。