Introduction

Alzheimer’s disease (AD) is the most common neurodegenerative disease across the world. The major cause of AD is extensive oxidative stress and inflammation in central nervous system (CNS). Vitamin D₃ and E are the most known vitamins that control oxidative stress and mitochondrial respiratory function. They may prevent neuronal loss in AD. Few studies have been conducted to assess the effect of vitamins on AD. Therefore, the aim of this study was to assess the effect of vitamin D₃ and E on AD. Methods: In this study, 60 rats were randomly divided into six groups (n = 10) namely the control group, sham group, AD group with intra-hippocampal Aβ1-40 injection, AD+vitamin D₃ group, AD+vitamin E group and AD+vitamin D₃ and E group. Memory and learning were measured by the Novel Object Recognition (NOR) test. Neuronal survival was assessed by H&E and cresyl violet staining, and oxidative stress was measured by malondialdehyde (MDA) level and superoxide dismutase (SOD) activity. In vitamin-treated groups, learning and memory, which were measured by NOR, improved significantly. Vitamin D₃ and E administration decreased neuronal loss in AD brain rats. Results: Results showed that MDA decreased and SOD increased in treatment groups; but, a combination of vitamin D₃ and E was more effective in decreasing oxidative stress in AD compared to other treatment groups. Conclusion: The present study indicated that vitamin D₃ and E and their combination can improve memory and learning deficit, and decrease neuronal loss and oxidative stress in Alzheimer’s model.

中文翻译：

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在患有阿尔茨海默氏病的大鼠模型中，服用维生素D3和E补充剂可减少神经元损失和氧化应激。

介绍

阿尔茨海默氏病（AD）是世界上最常见的神经退行性疾病。AD的主要原因是广泛的氧化应激和中枢神经系统（CNS）炎症。维生素D ₃和E是控制氧化应激和线粒体呼吸功能的最著名的维生素。它们可以预防AD的神经元丢失。很少有研究评估维生素对AD的影响。因此，本研究的目的是评估维生素D ₃和E对AD的影响。方法： 60只大鼠随机分为6组，每组10只，分别为对照组，假手术组，海马Aβ1-40注射液AD组，AD +维生素D ₃组，AD +维生素E组。和AD +维生素D ₃和E组。记忆和学习是通过新颖对象识别（NOR）测试进行的。通过H＆E和甲酚紫染色评估神经元存活，并通过丙二醛（MDA）水平和超氧化物歧化酶（SOD）活性测量氧化应激。在维生素治疗组中，通过NOR评估的学习和记忆能力显着改善。维生素D ₃和E的施用减少了AD脑大鼠的神经元丢失。结果：结果显示，治疗组的MDA降低而SOD升高。但是，与其他治疗组相比，维生素D ₃和E的组合在降低AD中的氧化应激方面更有效。结论：本研究表明维生素D ₃ E和它们的组合可以改善记忆力和学习障碍，并减少阿尔茨海默病模型中的神经元丢失和氧化应激。