Melanoma is an aggressive cancer with rapid progression, relapse, and metastasis. Systemic therapies for melanoma exhibit limited anticancer potential and high toxicity. Here, we developed the outer membrane vesicles derived from transgenic Escherichia coli, modified with α_vβ₃ integrin peptide targeting ligand and indocyanine green (named as I-P-OMVs), to induce the transdermal photo-TRAIL-programmed treatment in skin melanoma.-OMVs, which are outer membrane vesicles derived from transgenic Escherichia coli, modified with α_vβ₃ integrin targeting ligand and indocyanine green (named as I-P-OMVs), to induce the transdermal photo-TRAIL–programmed treatment in skin melanoma. I-P-OMVs exhibited excellent stratum corneum penetration and specificity to melanoma. Upon near-infrared irritation, I-P-OMVs not only induced photothermal-photodynamic responses against primary melanoma spheroids but also activated TRAIL-induced apoptosis in disseminated tumor cells, resulting in a complete eradication of melanoma. I-P-OMVs are the first nanoplatforms to induce transdermal photo-TRAIL–programmed therapy in melanoma with enhanced antitumor performance and high safety, having great potential in cancer therapy.

黑色素瘤是一种侵袭性癌症，进展迅速、复发和转移。黑色素瘤的全身疗法表现出有限的抗癌潜力和高毒性。在这里，我们开发了源自​​转基因大肠杆菌的外膜囊泡，并用α _v β ₃整合素肽靶向配体和吲哚菁绿（称为IP-OMV）进行修饰，以诱导皮肤黑色素瘤的透皮光TRAIL编程治疗。 OMV 是源自转基因大肠杆菌的外膜囊泡，用 α _v β ₃整合素靶向配体和吲哚菁绿（称为 IP-OMV）进行修饰，可诱导皮肤黑色素瘤的透皮光 TRAIL 程序治疗。IP-OMV 表现出优异的角质层渗透性和对黑色素瘤的特异性。在近红外刺激下，IP-OMV 不仅诱导针对原发性黑色素瘤球体的光热光动力反应，而且还激活 TRAIL 诱导的播散性肿瘤细胞凋亡，从而完全根除黑色素瘤。IP-OMV是第一个诱导黑色素瘤经皮光TRAIL程序治疗的纳米平台，具有增强的抗肿瘤性能和高安全性，在癌症治疗中具有巨大潜力。