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Ultrastructural investigation of acidocalcisomes and ATPase activity in yeasts Candida guillermondii NP‐4 as “complementary” stress‐targets
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-07-20 , DOI: 10.1111/lam.13348 K Hovnanyan 1 , S Marutyan 2 , S Marutyan 3 , M Hovnanyan 1 , L Navasardyan 2, 3 , A Trchounian 2, 3
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-07-20 , DOI: 10.1111/lam.13348 K Hovnanyan 1 , S Marutyan 2 , S Marutyan 3 , M Hovnanyan 1 , L Navasardyan 2, 3 , A Trchounian 2, 3
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As a result of electron microscopic studies of morphogenesis in yeast Candida guilliermondii NP‐4, the formation of new structures of volutin acidocalcisomes has been established within the cell cytoplasm. Under influence of X‐irradiation, the changes in morphometric and electron‐dense properties of yeast cells were identified: in yeast cytoplasm, the electron‐dense volutin granules were increased up to 400 nm in size. After 24‐h post‐irradiation incubation of yeasts, the large volutin pellets are fragmented into smaller number particles in size up to 25–150 nm. The ATPase activity in yeast mitochondria was changed under X‐irradiation. In latent phase of growth, ATPase activity was decreased 1·35‐fold in comparison with non‐irradiated yeasts. In logarithmic phase of growth, ATPase activity was three times higher than in latent phase, and in stationary phase of growth it has a value similar to the latent phase. Probably, the cells receive the necessary energy from alternative energy sources, such as volutin. Electron microscopy of volutin granule changes might serve as convenient method for evaluation of damages and repair processes in cells under influence of different environmental stress‐factors.
中文翻译：
酵母假丝酵母 NP-4 中酸性钙酶体和 ATP 酶活性的超微结构研究作为“补充”应激靶标
作为对酵母念珠菌 NP-4 形态发生的电子显微镜研究的结果，已在细胞质内形成了 volutin 酸钙体的新结构。在 X 辐射的影响下，酵母细胞的形态计量学和电子致密特性发生了变化：在酵母细胞质中，电子致密的 volutin 颗粒的大小增加到 400 nm。酵母辐照后 24 小时孵育后，大的 volutin 颗粒被破碎成较小的颗粒，尺寸可达 25-150 nm。酵母线粒体中的 ATPase 活性在 X 射线照射下发生变化。在生长的潜伏期，与未辐照的酵母相比，ATP 酶活性降低了 1·35 倍。在对数生长期，ATPase活性是潜伏期的三倍，并且在生长的稳定期它具有与潜伏期相似的值。可能，细胞从替代能源（例如 volutin）中获得必要的能量。volutin 颗粒变化的电子显微镜检查可能是评估不同环境应激因素影响下细胞损伤和修复过程的便捷方法。
更新日期：2020-07-20
中文翻译：
酵母假丝酵母 NP-4 中酸性钙酶体和 ATP 酶活性的超微结构研究作为“补充”应激靶标
作为对酵母念珠菌 NP-4 形态发生的电子显微镜研究的结果，已在细胞质内形成了 volutin 酸钙体的新结构。在 X 辐射的影响下，酵母细胞的形态计量学和电子致密特性发生了变化：在酵母细胞质中，电子致密的 volutin 颗粒的大小增加到 400 nm。酵母辐照后 24 小时孵育后，大的 volutin 颗粒被破碎成较小的颗粒，尺寸可达 25-150 nm。酵母线粒体中的 ATPase 活性在 X 射线照射下发生变化。在生长的潜伏期，与未辐照的酵母相比，ATP 酶活性降低了 1·35 倍。在对数生长期，ATPase活性是潜伏期的三倍，并且在生长的稳定期它具有与潜伏期相似的值。可能，细胞从替代能源（例如 volutin）中获得必要的能量。volutin 颗粒变化的电子显微镜检查可能是评估不同环境应激因素影响下细胞损伤和修复过程的便捷方法。
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