Functional analysis of Dicer-2 gene in Bombyx mori resistance to BmNPV virus.,Archives of Insect Biochemistry and Physiology

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Functional analysis of Dicer-2 gene in Bombyx mori resistance to BmNPV virus.
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2020-07-05 , DOI: 10.1002/arch.21724
Taohong Li ₁ , Yuchen Xia ₁ , Xinyue Xu ₁ , Guoqing Wei ₁ , Lei Wang ₁

Affiliation

Bombyx mori nucleopolyhedrovirus (BmNPV) is a primary pathogen in silkworm, and the molecular mechanism of B. mori defense to BmNPV infection is still unclear. RNA interference (RNAi) is well‐known as an intracellular conserved mechanism that is critical in gene regulation and cell defense. The antiviral RNAi pathway processes viral double‐stranded RNA (dsRNA) into viral small interfering RNAs that guide the recognition and cleavage of complementary viral target RNAs. In this study, a Dicer‐2 (Dcr2) gene was identified in B. mori and its antiviral function was explored. Dcr2 messenger RNA (mRNA) expression was the highest in hemocytes and expressed in all stages of silkworm growth. After infection with BmNPV, the expression of Dcr2 mRNA was significantly increased after infection in midgut and hemocytes. The expression of Dcr2 was significantly upregulated by injecting dsRNA (dsBmSPH‐1) into silkworm after 48 hr. Knocking down the expression level of Dcr2 using specific dsRNA in silkworm, which modestly enhanced the production of viral genomic DNA. Our results suggested that the Dcr2 gene in B. mori plays an important role in against BmNPV invasion.

中文翻译：

Dicer-2基因在家蚕对BmNPV病毒的抗性中的功能分析。

家蚕核多角体病毒（BmNPV）是家蚕中的主要病原体，而家蚕抗BmNPV感染的分子机制仍不清楚。RNA干扰（RNAi）是众所周知的一种细胞内保守机制，在基因调节和细胞防御中至关重要。抗病毒RNAi途径将病毒双链RNA（dsRNA）加工成病毒小干扰RNA，指导互补病毒靶RNA的识别和裂解。在这项研究中，在家蚕中发现了Dicer-2（Dcr2）基因，并探讨了其抗病毒功能。Dcr2信使RNA（mRNA）的表达在血细胞中最高，并在家蚕生长的所有阶段均表达。BmNPV感染后，中肠和血细胞感染后，Dcr2 mRNA的表达显着增加。通过在48小时后将dsRNA（dsBmSPH-1）注入家蚕，Dcr2的表达显着上调。使用特定的dsRNA抑制家蚕中Dcr2的表达水平，从而适度地提高了病毒基因组DNA的产生。我们的研究结果表明，在 DCR2基因家蚕抵抗入侵的BmNPV起着重要的作用。

更新日期：2020-07-05

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