Application of genotyping methods in the investigation of sources of dermatophytosis associated with vaccination in cattle,Annals of Applied Biology

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Application of genotyping methods in the investigation of sources of dermatophytosis associated with vaccination in cattle
Annals of Applied Biology ( IF 2.2 ) Pub Date : 2020-07-03 , DOI: 10.1111/aab.12618
Dominik Łagowski ₁ , Sebastian Gnat ₁ , Aneta Nowakiewicz ₁ , Marcelina Osińska ₁ , Przemysław Zięba ₂

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Vaccines against trichophytosis introduced over 60 years ago contributed to a significant reduction in the prevalence of dermatophyte infections on cattle farms. Currently, vaccination is not obligatory but commonly used and recommended by veterinarians, which is associated with the renaissance of dermatophyte infections in farm animals. Importantly, vaccine administration may cause clinical lesions imitating a virulent form of dermatophytosis. Correct determination of a possible correlation of superficial changes with the vaccine strain plays a key role in taking therapeutic measures. In this study, the usefulness of genotyping methods (melting‐point‐PCR and amplified fragment length polymorphism) in identification of the source of dermatophyte infection in cases correlated with vaccine administration is examined. The results revealed that dermatophytes isolated from skin lesions in calves temporarily associated with vaccine administration had an identical electrophoretic profile with the vaccine strain and differed from the reference strain of Trichophyton verrucosum CBS365.53. In conclusion, genotyping methods are a useful tool for rapid and reliable identification of the vaccine source of superficial lesions in calves. Their high resolution, speed of execution and low costs are undoubted advantages in the implementation for routine use in similar cases of dermatophyte infections.

中文翻译：

基因分型方法在牛皮肤病与疫苗接种相关来源调查中的应用

六十多年前推出的针对毛癣菌病的疫苗大大降低了牛场皮肤癣菌感染的发生率。目前，疫苗接种不是强制性的，而是兽医普遍使用和推荐的，这与农场动物皮肤癣菌感染的恢复有关。重要的是，接种疫苗可能会导致临床损伤，从而模仿出一种严重的皮肤癣菌病。正确确定表面变化与疫苗株之间可能的相关性在采取治疗措施中起着关键作用。在这项研究中，研究了基因分型方法（熔点PCR和扩增的片段长度多态性）在鉴定与疫苗接种有关的病例中皮肤癣菌感染来源的有用性。疣毛癣菌CBS365.53。总之，基因分型方法是一种快速，可靠地鉴定犊牛表皮病变疫苗来源的有用工具。它们的高分辨率，执行速度和低成本无疑是在类似皮肤癣菌感染病例中常规使用的实施中的优势。

更新日期：2020-07-03

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