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Characterization of Acidovorax citrulli strains isolated from solanaceous plants
Plant Pathology ( IF 2.3 ) Pub Date : 2020-07-04 , DOI: 10.1111/ppa.13239 Laura Chalupowicz 1 , Michal Reuven 1 , Orit Dror 1 , Noa Sela 1 , Saul Burdman 2 , Shulamit Manulis‐Sasson 1
Plant Pathology ( IF 2.3 ) Pub Date : 2020-07-04 , DOI: 10.1111/ppa.13239 Laura Chalupowicz 1 , Michal Reuven 1 , Orit Dror 1 , Noa Sela 1 , Saul Burdman 2 , Shulamit Manulis‐Sasson 1
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Acidovorax citrulli is the causal agent of bacterial fruit blotch disease of cucurbits. Strains of this pathogen are distributed into two major groups: Group I strains have been mainly isolated from melon and other non‐watermelon cucurbits, while Group II strains have been mainly recovered from watermelon. Here we report the characterization of strains T1 and EP isolated from diseased tomato and eggplant plants, respectively, and further confirmed to belong to A. citrulli species. Based on PCR, PFGE, and rep‐PCR, these strains showed high similarity to the Group II strain 7a1. Sequencing and comparative analyses revealed that the genomes of T1 and EP aligned with that of the Group II model strain AAC00‐1, over 97.88% and 99.22%, respectively. The virulence of T1, EP, and 7a1 determined on tomato, eggplant, and watermelon was similar and significantly higher than that of Group I strain M6. In contrast, M6 was more virulent on melon. Expression levels of seven virulence genes measured 24 hr after inoculation of tomato, eggplant, watermelon, and melon showed that the expression pattern was generally similar in strains 7a1, T1, and EP, whereas for M6 the expression was high only on melon. Overall, our results indicate that the solanaceous strains belong to Group II. To the best of our knowledge, this is the first study that reports characterization of A. citrulli strains isolated from solanaceous species. The fact that A. citrulli is able to naturally colonize and cause disease in non‐cucurbit crops poses additional challenges for management of this important pathogen.
中文翻译：
从茄科植物中分离到的Acidovorax citrulli菌株的特性
Acidovorax citrulli是葫芦科细菌性水果斑点病的病原体。该病原体的菌株分为两大类：第一类菌株主要从瓜类和其他非西瓜葫芦中分离出来，而第二类菌株主要从西瓜中回收。在这里，我们报告株T1和EP的分别从患病番茄和茄子植物，分离出的特征，并进一步证实属于一个。瓜特里种类。基于PCR，PFGE和rep-PCR，这些菌株与II组7a1具有高度相似性。测序和比较分析显示，T1和EP的基因组与II型模型菌株AAC00-1的基因组对齐，分别超过97.88％和99.22％。在番茄，茄子和西瓜上测得的T1，EP和7a1毒力相似，且显着高于I组M6菌株。相比之下，M6对甜瓜的毒性更大。接种番茄，茄子，西瓜和甜瓜后24小时测量的7个毒力基因的表达水平表明，菌株7a1，T1和EP中的表达模式通常相似，而对于M6，仅在甜瓜上表达较高。总的来说，我们的结果表明茄科菌株属于第二类。据我们所知，一。citrulli从茄科物种分离的菌株。A的事实。瓜果能够自然定殖并在非葫芦类作物中致病，这给这种重要病原体的管理带来了额外的挑战。
更新日期：2020-07-04
中文翻译：
从茄科植物中分离到的Acidovorax citrulli菌株的特性
Acidovorax citrulli是葫芦科细菌性水果斑点病的病原体。该病原体的菌株分为两大类：第一类菌株主要从瓜类和其他非西瓜葫芦中分离出来，而第二类菌株主要从西瓜中回收。在这里，我们报告株T1和EP的分别从患病番茄和茄子植物，分离出的特征，并进一步证实属于一个。瓜特里种类。基于PCR，PFGE和rep-PCR，这些菌株与II组7a1具有高度相似性。测序和比较分析显示，T1和EP的基因组与II型模型菌株AAC00-1的基因组对齐，分别超过97.88％和99.22％。在番茄，茄子和西瓜上测得的T1，EP和7a1毒力相似，且显着高于I组M6菌株。相比之下，M6对甜瓜的毒性更大。接种番茄，茄子，西瓜和甜瓜后24小时测量的7个毒力基因的表达水平表明，菌株7a1，T1和EP中的表达模式通常相似，而对于M6，仅在甜瓜上表达较高。总的来说，我们的结果表明茄科菌株属于第二类。据我们所知，一。citrulli从茄科物种分离的菌株。A的事实。瓜果能够自然定殖并在非葫芦类作物中致病，这给这种重要病原体的管理带来了额外的挑战。
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