Imaging mass spectrometry (IMS) technologies are capable of mapping a wide array of biomolecules in diverse cellular and tissue environments. IMS has emerged as an essential tool for providing spatially targeted molecular information due to its high sensitivity, wide molecular coverage, and chemical specificity. One of the major challenges for mapping the complex cellular milieu is the presence of many isomers and isobars in these samples. This challenge is traditionally addressed using orthogonal liquid chromatography (LC)‐based analysis, though, common approaches such as chromatography and electrophoresis are not able to be performed at timescales that are compatible with most imaging applications. Ion mobility offers rapid, gas‐phase separations that are readily integrated with IMS workflows in order to provide additional data dimensionality that can improve signal‐to‐noise, dynamic range, and specificity. Here, we highlight recent examples of ion mobility coupled to IMS and highlight their importance to the field.

中文翻译：

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整合离子迁移率和成像质谱法对生物组织进行综合分析：简要回顾和展望。

成像质谱 (IMS) 技术能够在不同的细胞和组织环境中绘制各种生物分子。由于其高灵敏度、广泛的分子覆盖范围和化学特异性，IMS 已成为提供空间靶向分子信息的重要工具。绘制复杂细胞环境的主要挑战之一是这些样品中存在许多异构体和同量异位素。传统上使用基于正交液相色谱 (LC) 的分析来解决这一挑战，但是，色谱和电泳等常用方法无法在与大多数成像应用兼容的时间尺度上进行。离子淌度提供快速、易于与 IMS 工作流程集成的气相分离，以提供额外的数据维度，从而提高信噪比、动态范围和特异性。在这里，我们重点介绍了离子迁移率与 IMS 耦合的最新示例，并强调了它们对该领域的重要性。