Changes in the microstructure and enzymatic hydrolysis performance of chitin treated by steam explosion, high‐pressure homogenization, and γ radiation,Journal of Applied Polymer Science

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Changes in the microstructure and enzymatic hydrolysis performance of chitin treated by steam explosion, high‐pressure homogenization, and γ radiation
Journal of Applied Polymer Science ( IF 2.7 ) Pub Date : 2020-07-03 , DOI: 10.1002/app.49597
Miaomiao Zhai _{1,

2} , Jinghe Du _{1,

2,

3} , Jun Zhang ₄ , Jianyin Miao _{1,

2} , Jianglong Luo ₄ , Yong Cao _{1,

2} , Shan Duan _{1,

2}

Affiliation

This research intends to reduce the crystallinity of chitin with physical methods so as to improve the enzymatic hydrolysis efficiency of chitin. Scanning electron microscopy showed that both steam explosion and high‐pressure homogenization significantly enlarged the pores in shrimp shells, which are made up of chitin, but γ radiation did not. X‐ray diffraction showed that the crystallinity index of chitin decreased by 23.2% in the (110) plane and 24.7% in the (020) plane after three rounds of steam explosion and decreased by 12.4% in the (110) plane and 28.7% in the (020) plane after three rounds of high‐pressure homogenization. After γ radiation, however, there was no significant change. The corresponding deacetylation degrees of the chitin treated with steam explosion and high‐pressure homogenization increased by 13.2 and 5.7%, respectively. The natural, steam‐exploded and high‐pressure homogenized chitins respectively produced 0.251, 0.145, and 0.407 mg/ml reducing sugars with the hydrolysis of Streptomyces griseus chitinase. However, when hydrolyzed by Paenibacillus sp. A1 chitinase the steam‐exploded and the high‐pressure homogenized chitin respectively released 40 and 300% more reducing sugars than the natural chitin. The results found that steam explosion and high‐pressure homogenization can reduce the crystallinity of chitin, and make it prone to enzymatic hydrolysis.

中文翻译：

蒸汽爆炸，高压均质化和γ辐射处理后的几丁质的微观结构和酶水解性能的变化

本研究旨在通过物理方法降低几丁质的结晶度，以提高几丁质的酶水解效率。扫描电子显微镜显示，蒸汽爆炸和高压均质化均显着扩大了由甲壳质组成的虾壳中的孔，但γ射线没有。X射线衍射显示，经过三轮蒸汽爆炸后，几丁质的结晶度指数在（110）平面下降23.2％，在（020）平面下降24.7％，在（110）平面下降12.4％，在28.7％下降经过三轮高压均质后，在（020）平面中旋转。但是，在γ辐射后，没有明显变化。蒸汽爆炸法和高压均质法处理的几丁质相应的脱乙酰度分别增加了13.2和5.7％。灰链霉菌几丁质酶。但是，当被Paenibacillus sp。水解时。蒸汽爆炸的甲壳质几丁质酶和高压均质甲壳质分别比天然甲壳质多释放40％和300％的还原糖。结果发现，蒸汽爆炸和高压均质化可降低甲壳质的结晶度，并使其易于酶水解。

更新日期：2020-07-03

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