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Resistance to succinate dehydrogenase inhibitors in field isolates of Podosphaera xanthii on cucumber: Monitoring, cross-resistance patterns and molecular characterization
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.pestbp.2020.104646
Takuya Miyamoto 1 , Kanako Hayashi 1 , Ryo Okada 1 , David Wari 1 , Takashi Ogawara 1
Affiliation  

New succinate dehydrogenase inhibitor fungicides (SDHIs), isopyrazam, pyraziflumid and isofetamid were introduced in the Japanese market in 2017-2018 to control powdery mildew on cucumber. SDHI resistance of the disease fungus (Podosphaera xanthii) was first reported during 2008-2009 against boscalid. Then, penthiopyrad which belongs to SDHIs was introduced in 2010, but subsequent monitoring study was not performed. We investigated the sensitivity of P. xanthii field isolates from Ibaraki Prefecture, Japan, to SDHIs and SdhB, SdhC and SdhD gene mutations, using a leaf disc assay and SDH gene analysis. A total of 19 out of the 22 selected isolates showed resistance to SDHIs. The 19 isolates were phenotypically categorized into three types: Resistant I as moderately and Resistant II as highly resistant to penthiopyrad, isopyrazam and pyraziflumid but sensitive to isofetamid and Resistant III as highly resistant to isofetamid but sensitive to the other three SDHIs. SDH gene analysis revealed that Resistant I and III isolates carried a substitution in PxD-S121P and PxC-A86V, respectively. Resistant II carried three different substitutions: PxC-G151R, PxC-G172D, and PxD-H137R. Among 127 isolates sampled from 16 cucumber greenhouses, 54 exhibited Resistant I phenotype and carried only PxD-S121P. Fifty-six isolates exhibited Resistant II and carried PxC-G151R (four isolates), PxC-G172D (24), and PxD-H137R (28). Only two isolates expressed the Resistant III phenotype carrying PxC-A86V. To the best of our knowledge, this is the first report demonstrating cross-resistance patterns and the molecular characterization of SDHIs in P. xanthii.

中文翻译:

黄瓜上的 Podosphaera xanthii 田间分离株对琥珀酸脱氢酶抑制剂的抗性:监测、交叉抗性模式和分子表征

2017-2018 年,日本市场推出了新型琥珀酸脱氢酶抑制剂杀菌剂 (SDHI)、异吡唑啉、吡嗪酰胺和异噻菌胺,以控制黄瓜上的白粉病。2008-2009 年期间首次报道了疾病真菌(Podosphaera xanthii)对啶酰菌胺的 SDHI 抗性。2010年又引进了属于SDHIs的吡噻菌胺,但没有进行后续的监测研究。我们使用叶盘测定和 SDH 基因分析研究了来自日本茨城县的 P. xanthii 野外分离物对 SDHI 和 SdhB、SdhC 和 SdhD 基因突变的敏感性。在 22 个选定的分离株中,共有 19 个显示出对 SDHI 的抗性。这 19 株分离株在表型上分为三类:抗性 I 为中等,抗性 II 为高度抗吡噻菌胺,isopyrazam和pyraziflumid,但对isofetamid和Resistance III敏感,对isofetamid具有高度耐药性,但对其他三种SDHIs敏感。SDH 基因分析显示,抗性 I 和 III 分离株分别在 PxD-S121P 和 PxC-A86V 中进行了替换。抗性 II 带有三种不同的取代:PxC-G151R、PxC-G172D 和 PxD-H137R。在从 16 个黄瓜温室采样的 127 个分离株中,54 个表现出抗性 I 表型,仅携带 PxD-S121P。56 个分离株表现出抗性 II 并携带 PxC-G151R(4 个分离株)、PxC-G172D(24 个)和 PxD-H137R(28 个)。只有两个分离株表达了携带 PxC-A86V 的抗性 III 表型。据我们所知,这是第一份展示交叉抗性模式和 P. xanthii 中 SDHI 分子特征的报告。
更新日期:2020-10-01
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