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Multianalyte electrochemical electrode for the determination of vitamins B2 and B6 in complex biosystem
Microchemical Journal ( IF 4.9 ) Pub Date : 2020-11-01 , DOI: 10.1016/j.microc.2020.105233
Huimin Wang , Xueli Zhang , Shuangjue Wang , Sa Xiao , Hanyue Ma , Xia Wang

Abstract Vitamin B2 (VB2) and vitamin B6 (VB6) as important vitamins for human health are mainly produced by industrial microorganism fermentation. Due to the complexity of fermentation system, there is a lack of an efficient, rapid, and convenient detection method in the fermentation production of these two vitamins. In this study, a multianalyte electrochemical electrode based on glassy carbon electrode (GCE) modified by nanoporous gold (NPG) film was developed. This is the first time that NPG realized the electrochemical detection of VB2 and VB6 in complex fermentation analysis. Under optimized conditions, the NPG-modified electrode exhibited good performance in VB2 and VB6 detection using differential pulse voltammetry (DPV). Two linear responses in wide concentration ranges for VB2 from 5 to 250 μM and for VB6 from 40 to 1000 μM were obtained with the limit of detection (LOD) of 0.1 μM and 0.4 μM as well as the high sensitivities of 178.02 μA mM−1 cm−2 and 43.14 μA mM−1 cm−2, respectively. Furthermore, the detection of VB2 and VB6 in fermentation media was successfully achieved using the NPG-modified electrode with high sensitivity and strong anti-interference ability. Additionally, the NPG-modified electrode has a long life because of the metal characteristics of NPG. These above characteristics conformed that the multianalyte electrochemical electrode can realize the simultaneous detection of multiple substances such as substrates and products in a complex biosystem.

中文翻译:

用于测定复杂生物系统中维生素 B2 和 B6 的多分析物电化学电极

摘要 维生素B2(VB2)和维生素B6(VB6)作为人类健康重要的维生素,主要由工业微生物发酵生产。由于发酵系统的复杂性,这两种维生素的发酵生产缺乏一种高效、快速、方便的检测方法。在这项研究中,开发了一种基于纳米多孔金 (NPG) 膜修饰的玻璃碳电极 (GCE) 的多分析物电化学电极。这是NPG首次在复杂发酵分析中实现VB2和VB6的电化学检测。在优化条件下,NPG 修饰电极在使用差分脉冲伏安法 (DPV) 检测 VB2 和 VB6 时表现出良好的性能。在 5 至 250 μM 的 VB2 和 40 至 1000 μM 的 VB6 的宽浓度范围内获得了两个线性响应,检测限 (LOD) 为 0.1 μM 和 0.4 μM,灵敏度为 178.02 μA mM-1 cm-2 和 43.14 μA mM-1 cm-2,分别。此外,利用灵敏度高、抗干扰能力强的NPG修饰电极,成功实现了发酵培养基中VB2和VB6的检测。此外,由于 NPG 的金属特性,NPG 改性电极具有较长的寿命。上述特点表明,多分析物电化学电极可以实现复杂生物系统中多种物质如底物和产物的同时检测。分别为 02 μA mM-1 cm-2 和 43.14 μA mM-1 cm-2。此外,利用灵敏度高、抗干扰能力强的NPG修饰电极,成功实现了发酵培养基中VB2和VB6的检测。此外,由于 NPG 的金属特性,NPG 改性电极具有较长的寿命。上述特点表明,多分析物电化学电极可以实现复杂生物系统中多种物质如底物和产物的同时检测。分别为 02 μA mM-1 cm-2 和 43.14 μA mM-1 cm-2。此外,利用灵敏度高、抗干扰能力强的NPG修饰电极,成功实现了发酵培养基中VB2和VB6的检测。此外,由于 NPG 的金属特性,NPG 改性电极具有较长的寿命。上述特点表明,多分析物电化学电极可以实现复杂生物系统中多种物质如底物和产物的同时检测。由于 NPG 的金属特性,NPG 改性电极具有较长的寿命。上述特点表明,多分析物电化学电极可以实现复杂生物系统中多种物质如底物和产物的同时检测。由于 NPG 的金属特性,NPG 改性电极具有较长的寿命。上述特点表明,多分析物电化学电极可以实现复杂生物系统中多种物质如底物和产物的同时检测。
更新日期:2020-11-01
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