Many studies on beef nutritional qualities require the quantification of intramuscular fat. To reduce the sample amount, solvent use and time of analysis, two alternative methods to the Folch et al. (1957) reference method were studied: a miniaturised Folch's method and a near-infrared spectroscopic method. Performances and acceptability limits were evaluated with accuracy profiles for each of the methods. Equations to correct bias between the alternative and reference methods were calculated. Uncertainties associated with measurements were determined, and the validity domains were defined. From a previous set of studies, the ability of each method to discriminate samples from bovines of different breeds or receiving diverse treatments was tested. The validity domain of the miniaturised Folch's method ranged from 1.9 to 13.8 g of total lipids/100 g of tissue, and that of the near-infrared spectroscopic method ranged from 4.8 to 13.8 g of total lipids/100 g of tissue, with less than 20% difference from the reference method's results. Thus, the two alternative methods could be used depending on the research objectives: the miniaturised Folch's method could be used for detailed quantification of intramuscular fat and the near-infrared spectroscopic method for a quick classification of a large number of muscles. The precise knowledge of uncertainties associated with each measurement was determined, and perfect continuity with the results obtained so far with the reference Folch's method was confirmed.

中文翻译：

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两种牛肉肌内脂肪定量实验室方法的验证

许多关于牛肉营养品质的研究需要对肌内脂肪进行量化。为了减少样品量、溶剂使用和分析时间，Folch 等人的两种替代方法。(1957) 研究了参考方法：小型化的 Folch 方法和近红外光谱方法。使用每种方法的准确度曲线评估性能和可接受限度。计算了用于校正替代方法和参考方法之间偏差的方程。确定了与测量相关的不确定性，并定义了有效性域。从之前的一组研究中，测试了每种方法区分来自不同品种或接受不同处理的牛的样本的能力。小型化 Folch 方法的有效域范围为 1.9 到 13。8 g 总脂质/100 g 组织，近红外光谱法的总脂质为 4.8 至 13.8 g/100 g 组织，与参考方法的结果相差不到 20%。因此，可以根据研究目标使用两种替代方法：小型 Folch 方法可用于详细量化肌内脂肪，近红外光谱法可用于快速分类大量肌肉。确定了与每次测量相关的不确定性的精确知识，并确认了迄今为止使用参考 Folch 方法获得的结果的完美连续性。与参考方法结果的差异小于 20%。因此，可以根据研究目标使用两种替代方法：小型 Folch 方法可用于详细量化肌内脂肪，近红外光谱法可用于快速分类大量肌肉。确定了与每次测量相关的不确定性的精确知识，并确认了迄今为止使用参考 Folch 方法获得的结果的完美连续性。与参考方法结果的差异小于 20%。因此，可以根据研究目标使用两种替代方法：小型 Folch 方法可用于详细量化肌内脂肪，近红外光谱法可用于快速分类大量肌肉。确定了与每次测量相关的不确定性的精确知识，并确认了迄今为止使用参考 Folch 方法获得的结果的完美连续性。s 方法可用于肌肉内脂肪的详细量化，近红外光谱方法可用于快速分类大量肌肉。确定了与每次测量相关的不确定性的精确知识，并确认了迄今为止使用参考 Folch 方法获得的结果的完美连续性。s 方法可用于肌肉内脂肪的详细量化，近红外光谱方法可用于快速分类大量肌肉。确定了与每次测量相关的不确定性的精确知识，并确认了迄今为止使用参考 Folch 方法获得的结果的完美连续性。