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Top down proteomic analysis of gingival crevicular fluid in deciduous, exfoliating and permanent teeth in children.
Journal of Proteomics ( IF 2.8 ) Pub Date : 2020-07-03 , DOI: 10.1016/j.jprot.2020.103890
Federica Iavarone 1 , Alessandra Olianas 2 , Romeo Patini 3 , Patrizia Gallenzi 3 , Laura Di Tonno 3 , Claudia Desiderio 4 , Tiziana Cabras 2 , Barbara Manconi 2 , Federica Vincenzoni 1 , Massimo Cordaro 3 , Irene Messana 4 , Andrea Urbani 1 , Massimo Castagnola 5
Affiliation  

Gingival Crevicular Fluid (GCF), a plasma-derived exudate present in the gingival crevice was collected from deciduous, exfoliating and permanent teeth from 20 children (60 samples) with the aim to characterize and quantify by a mass spectrometry based top-down proteomic approach, the peptide/proteins in the fluid and verify possible variations occurring during the exfoliating process.

The results obtained confirmed the presence in GCF of α-Defensins 1–4, Thymosin β4 and Thymosin β10, as described in previous works and revealed the presence of other interesting peptides never described before in GCF such as specific fragments of α-1-antitrypsin, α-1-antichymotrypsin; fragments of Thymosin β4 and Thymosin β10; Fibrinopeptide A and its fragments and Fibrinopeptide B; S100A8 and S100A9, LVV Hemorphin-7 (hemoglobin chain β fragment), as well as some other peptides deriving from α and β subunits of hemoglobin. Statistical analysis evidenced different levels in 5 proteins/peptides in the three groups. Our study demonstrate that an in-depth analysis of a biological fluid like GCF, present in small amount, can provide useful information for the understanding of different biological processes like teeth eruption. Data are available via ProteomeXchange with identifier PXD016010 and PXD016049.

Significance

GCF due to his site-specific nature has a great potential in containing factors that are specific for action at a given site and might have diagnostic value to detect qualitative and quantitative variations of proteins/peptides composition linked to physiological or pathological conditions.



中文翻译:

自上而下的蛋白质组学分析儿童乳牙,脱落和恒牙的龈沟液。

牙龈沟液(GCF)是牙龈缝隙中血浆衍生的渗出液,是从20名儿童(60个样本)的落叶,脱落和永久性牙齿中收集的,目的是通过基于质谱的自上而下的蛋白质组学方法进行表征和定量,液体中的肽/蛋白质,并验证剥离过程中可能发生的变化。

如先前工作所述,获得的结果证实了α-防御素1-4,胸腺素β4和胸腺素β10在GCF中的存在,并揭示了在GCF中从未存在过的其他有趣的肽,例如α-1-抗胰蛋白酶的特定片段。 ,α-1-抗胰凝乳蛋白酶;胸腺素β4和胸腺素β10的片段;纤维蛋白肽A及其片段和纤维蛋白肽B;S100A8和S100A9,LVV Hemorphin-7(血红蛋白链β片段)以及其他一些来自血红蛋白α和β亚基的肽。统计分析表明,三组中的5种蛋白质/肽水平不同。我们的研究表明,对少量存在的生物流体(如GCF)进行深入分析,可以为了解不同的生物过程(如牙齿萌出)提供有用的信息。

意义

GCF由于其位点特异性的性质,在包含特定于给定位点作用的因子方面具有巨大潜力,并且可能具有诊断价值,可检测与生理或病理状况相关的蛋白质/肽组成的定性和定量变化。

更新日期:2020-07-13
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