The utilization of swine wastewater is affected by salinity and pH owing to the extensive use with seawater instead of domestic water as swine farm flushing water in coastal city. Therefore, swine wastewater pretreated with thermophilic bacteria was used as fermentation substrate in this work, the effects of salinity and pH on dark fermentation under mesophilic condition were investigated. The research showed that 1.5% salinity and pH 6.0 were the optimal conditions for hydrogen production with swine wastewater. The activity of hydrogenogen was inhibited at 3.5% salinity and pH 5.0. Soluble organic matter in substrate was accumulated under high salinity and alkaline conditions. The utilization of carbohydrate during dark fermentation was up to 61.1% at 1.5% salinity and 51.5% at pH 9.0. Enhancing of salinity and pH had an advantage in accumulation of total soluble metabolites. Acetate was the main metabolite during dark fermentation, and 1.5% salinity contributed to the formation of butyrate.

猪废水的利用受到盐度和pH值的影响，这是因为在沿海城市，广泛使用海水代替了生活用水作为养猪场的冲洗水。因此，以嗜热菌预处理的猪废水为发酵底物，研究了盐度和pH值对中温条件下黑暗发酵的影响。研究表明，1.5％的盐度和pH 6.0是猪场废水制氢的最佳条件。在3.5％的盐度和pH 5.0时，氢气的活性受到抑制。基质中的可溶性有机物在高盐度和碱性条件下积累。在盐度为1.5％时，黑暗发酵过程中碳水化合物的利用率最高为61.1％，在pH值为9.0时，碳水化合物的利用率高达51.5％。盐度和pH值的提高在总可溶性代谢物积累方面具有优势。乙酸盐是深色发酵过程中的主要代谢产物，盐度为1.5％有助于形成丁酸盐。