NAC is one of the largest family of plant-specific transcription factors, and it plays important roles in plant development and stress responses. The study identified 72 LpNACs genes from the perennial ryegrass genome database. Gene length, MW and pI of NAC family transcription factors varied, but the gene structure and motifs were relatively conserved in bioinformatics analysis. Phylogenetic analyses of perennial ryegrass, rice and Arabidopsis were performed to study the evolutionary and functional relationships in various species. The expression of LpNAC genes that respond to various abiotic stresses including high salinity, ABA, high temperature, polyethylene glycol (PEG) and heavy metal was comprehensively analyzed. The present study provides a basic understanding of the NAC gene family in perennial ryegrass for further abiotic stress studies and improvements in breeding.

NAC 是最大的植物特异性转录因子家族之一，在植物发育和逆境响应中发挥重要作用。该研究从多年生黑麦草基因组数据库中鉴定了 72 个 LpNACs 基因。NAC 家族转录因子的基因长度、MW 和 pI 各不相同，但基因结构和基序在生物信息学分析中相对保守。对多年生黑麦草、水稻和拟南芥进行系统发育分析，以研究不同物种的进化和功能关系。LpNAC的表达综合分析了响应各种非生物胁迫的基因，包括高盐度、ABA、高温、聚乙二醇（PEG）和重金属。本研究为进一步的非生物胁迫研究和育种改进提供了对多年生黑麦草 NAC 基因家族的基本了解。